A1 Journal article (refereed)
Low Levels of Mitochondrial DNA and Symbiont Diversity in the Worldwide Agricultural Pest, the Greenhouse Whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) (2015)

Kapantaidaki, D. E., Ovcarenko, I., Fytrou, N., Knott, E., Bourtzis, K., & Tsagkarakou, A. (2015). Low Levels of Mitochondrial DNA and Symbiont Diversity in the Worldwide Agricultural Pest, the Greenhouse Whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae). Journal of Heredity, 106(1), 80-92. https://doi.org/10.1093/jhered/esu061

JYU authors or editors

Publication details

All authors or editors: Kapantaidaki, Despoina E.; Ovcarenko, Irina; Fytrou, Natasa; Knott, Emily; Bourtzis, Kostas; Tsagkarakou, Anastasia

Journal or series: Journal of Heredity

ISSN: 0022-1503

eISSN: 1465-7333

Publication year: 2015

Volume: 106

Issue number: 1

Pages range: 80-92

Publisher: Oxford University Press; American Genetic Association

Publication country: United Kingdom

Publication language: English

DOI: https://doi.org/10.1093/jhered/esu061

Publication open access: Not open

Publication channel open access: 

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/44853

Abstract

Trialeurodes vaporariorum, the greenhouse whitefly, is a cosmopolitan agricultural pest. Little is known about the genetic diversity of T. vaporariorum and the bacterial symbionts associated with this species. Here, we undertook a large phylogeographic study by investigating both the mitochondrial (mt) diversity and the infection status of 38 T. vaporariorum collections from 18 countries around the world. Genetic diversity of T. vaporariorum was studied by analyzing sequence data from the mt cytochrome oxidase I, cytochrome b, and NADH dehydrogenase subunit 5 genes. Maximum-likelihood (ML) phylogeny reconstruction delineated 2 clades characterized by limited sequence divergence: one clade comprised samples only from the Northern hemisphere whereas the other comprised samples from a broader geographical range. The presence of secondary symbionts was determined by PCR using primers specific for Hamiltonella, Rickettsia, Arsenophonus, Cardinium, Wolbachia, and Fritschea. Most individuals examined harbored at least one secondary endosymbiont, and Arsenophonus was detected in almost all male and female individuals. Wolbachia was present at a much lower frequency, and Cardinium was detected in only a few individuals from Greece. Rickettsia, Hamiltonella, and Fritschea were not found. Additionally, we set out to further analyze Arsenophonus diversity by multilocus sequence typing analysis; however, the Arsenophonus sequences did not exhibit any polymorphism. Our results revealed remarkably low diversity in both mtDNA and symbionts in this worldwide agricultural pest, contrasting sharply with that of the ecologically similar Bemisia tabaci.

Free keywords: Arsenophonus; genetic diversity; mtDNA polymorphism; symbiont communities

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Ministry reporting: Yes

Reporting Year: 2015

JUFO rating: 1