CRISPR and antagonistic coevolution with bacterial viruses – linking molecular evolution of both host and its parasite
Main funder
Funder's project number: 314939
Funds granted by main funder (€)
- 515 568,00
Funding program
Project timetable
Project start date: 01/09/2018
Project end date: 31/08/2022
Summary
Despite much excitement regarding the applied aspects of CRISPR, there remain numerous unresolved questions regarding 1) diversity and dynamics of CRISPR adaptation, 2) possible trade-offs between CRISPR mediated immunity and other bacterial life-history traits, 3) respective genetic adaptation in antagonistic coevolution between viruses and bacteria. I will use a phage-bacterium system to explore the functionality and diversity of host adaptive immunity both at molecular and phenotypic levels. I will also study the coevolutionary effects on the virus targeted by the bacterial immunity. This project will fill a gap in knowledge concerning overlapping coevolution of hosts and pathogens, especially outside laboratory conditions and provide central information in developing virus-based applications to tackle diseases at the current post-antibiotic era.
Principal Investigator
Primary responsible unit
Internal follow-up group
Related publications and other outputs
- Cryo-EM structure of ssDNA bacteriophage ΦCjT23 provides insight into early virus evolution (2022) Kejzar, Nejc; et al.; A1; OA
- Genetic-based evaluation of management units for sustainable vendace (Coregonus albula) fisheries in a large lake system (2022) Karjalainen, Juha; et al.; A1; OA
- Mucin induces CRISPR-Cas defense in an opportunistic pathogen (2022) de Freitas Almeida, Gabriel Magno; et al.; A1; OA
- Prevalence of genetically similar Flavobacterium columnare phages across aquaculture environments reveals a strong potential for pathogen control (2022) Runtuvuori‐Salmela, A.; et al.; A1; OA
- Rich resource environment of fish farms facilitates phenotypic variation and virulence in an opportunistic fish pathogen (2022) Pulkkinen, Katja; et al.; A1; OA
- Bacteriophage Resistance Affects Flavobacterium columnare Virulence Partly via Mutations in Genes Related to Gliding Motility and Type IX Secretion System (2021) Kunttu, Heidi M. T.; et al.; A1; OA
- Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout (2021) Kunttu, Heidi M. T.; et al.; A1; OA
- Cooperation between Different CRISPR-Cas Types Enables Adaptation in an RNA-Targeting System (2021) Hoikkala, Ville; et al.; A1; OA
- Interactions between Rainbow Trout Eyed Eggs and Flavobacterium spp. Using a Bath Challenge Model : Preliminary Evaluation of Bacteriophages as Pathogen Control Agents (2021) Donati, Valentina L.; et al.; A1; OA
- Opposing health effects of hybridization for conservation (2021) Klemme, Ines; et al.; A1; OA
Related research datasets
- Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout (2021) Kunttu, Heidi; et al.
- Dataset for Mucin induces CRISPR-Cas defense in an opportunistic pathogen (2022) Almeida, Gabriel MF; et al.
- Original data for manuscript: Quantity and Quality of Aquaculture Enrichments Influence Disease Epidemics and Provide Ecological Alternatives to Antibiotics (2021) Karvonen, Anssi; et al.; URN:NBN:fi:jyu-202103222041;10.17011/jyx/dataset/74704
