A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
A molecular‐based identification resource for the arthropods of Finland (2022)
Roslin, T., Somervuo, P., Pentinsaari, M., Hebert, P. D. N., Agda, J., Ahlroth, P., Anttonen, P., Aspi, J., Blagoev, G., Blanco, S., Chan, D., Clayhills, T., deWaard, J., deWaard, S., Elliot, T., Elo, R., Haapala, S., Helve, E., Ilmonen, J., . . . Mutanen, M. (2022). A molecular‐based identification resource for the arthropods of Finland. Molecular Ecology Resources, 22(2), 803-922. https://doi.org/10.1111/1755-0998.13510
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Roslin, Tomas; Somervuo, Panu; Pentinsaari, Mikko; Hebert, Paul D. N.; Agda, Jireh; Ahlroth, Petri; Anttonen, Perttu; Aspi, Jouni; Blagoev, Gergin; Blanco, Santiago; et al.
Lehti tai sarja: Molecular Ecology Resources
ISSN: 1755-098X
eISSN: 1755-0998
Julkaisuvuosi: 2022
Ilmestymispäivä: 25.09.2021
Volyymi: 22
Lehden numero: 2
Artikkelin sivunumerot: 803-922
Kustantaja: Wiley-Blackwell
Julkaisumaa: Britannia
Julkaisun kieli: englanti
DOI: https://doi.org/10.1111/1755-0998.13510
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/77955
Rinnakkaistallenteen verkko-osoite (pre-print): https://www.authorea.com/doi/full/10.22541/au.162245457.73290867
Tiivistelmä
To associate specimens identified by molecular characters to other biological knowledge, we need reference sequences annotated by Linnaean taxonomy. In this paper, we 1) report the creation of a comprehensive reference library of DNA barcodes for the arthropods of an entire country (Finland), 2) publish this library, and 3) deliver a new identification tool for insects and spiders, as based on this resource. The reference library contains mtDNA COI barcodes for 11,275 (43%) of 26,437 arthropod species known from Finland, including 10,811 (45%) of 23,956 insect species. To quantify the improvement in identification accuracy enabled by the current reference library, we ran 1,000 Finnish insect and spider species through the Barcode of Life Data system (BOLD) identification engine. Of these, 91% were correctly assigned to a unique species when compared to the new reference library alone, 85% were correctly identified when compared to BOLD with the new material included, and 75% with the new material excluded. To capitalize on this resource, we used the new reference material to train a probabilistic taxonomic assignment tool, FinPROTAX, scoring high success. For the full-length barcode region, the accuracy of taxonomic assignments at the level of classes, orders, families, subfamilies, tribes, genera, and species reached 99.9%, 99.9%, 99.8%, 99.7%, 99.4%, 96.8%, and 88.5%, respectively. The FinBOL arthropod reference library and FinPROTAX are available through the Finnish Biodiversity Information Facility (www.laji.fi). Overall, the FinBOL investment represents a massive capacity-transfer from the taxonomic community of Finland to all sectors of society.
YSO-asiasanat: systematiikka (biologia); lajinmääritys; DNA-analyysi; niveljalkaiset
Vapaat asiasanat: arthropods; identification
Liittyvät organisaatiot
OKM-raportointi: Kyllä
Raportointivuosi: 2022
JUFO-taso: 1
