A1 Journal article (refereed)
A DNA‐Encoded FRET Biosensor for Visualizing the Tension across Paxillin in Living Cells upon Shear Stress (2022)

Shao, S., Deng, S., Jiang, Q., Zhang, H., Zhang, Z., Li, N., Cong, F., Tiihonen, T., & Liu, B. (2022). A DNA‐Encoded FRET Biosensor for Visualizing the Tension across Paxillin in Living Cells upon Shear Stress. Analysis and Sensing, 2(1), Article e202100033. https://doi.org/10.1002/anse.202100033

JYU authors or editors

Publication details

All authors or editors: Shao, Shuai; Deng, Sha; Jiang, Qingyun; Zhang, Hangyu; Zhang, Zhengyao; Li, Na; Cong, Fengyu; Tiihonen, Timo; Liu, Bo

Journal or series: Analysis and Sensing

eISSN: 2629-2742

Publication year: 2022

Publication date: 13/10/2021

Volume: 2

Issue number: 1

Article number: e202100033

Publisher: Wiley

Publication country: Germany

Publication language: English

DOI: https://doi.org/10.1002/anse.202100033

Publication open access: Not open

Publication channel open access:

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/78225


Paxillin is a potential participant in the direct intracellular force transmission which is considered as the foundation of cells sensing and responding to extracellular environment. However, the detection of tension across paxillin has not been achieved due to lacking microsized tools. Herein, a paxillin tension sensor (PaxTs) based on Fluorescence Resonance Energy Transfer (FRET) technique was constructed. PaxTs can be expressed and assembled to FA sites spontaneously to visualize the tension across paxillin with FRET efficiency of ~62.4% in living cells. The tension across paxillin was found to decrease upon shear stress, in which the membrane fluidity and contractility of actin acted as cushions. It is observed that paxillin participates in the pathway of cell membrane-cytoskeleton-FAs for force transmission upon mechanical force in real time visualization, which provides a promising new method to investigate the direct intracellular force transmission in biology and technology.

Keywords: biosensors; cell physiology; cell signaling; proteins; mechanics

Free keywords: biosensors; focal adhesions; FRET; paxillin; shear stress

Contributing organizations

Ministry reporting: Yes

Reporting Year: 2022

Last updated on 2022-20-09 at 14:47