A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
A DNA‐Encoded FRET Biosensor for Visualizing the Tension across Paxillin in Living Cells upon Shear Stress (2022)

Shao, S., Deng, S., Jiang, Q., Zhang, H., Zhang, Z., Li, N., Cong, F., Tiihonen, T., & Liu, B. (2022). A DNA‐Encoded FRET Biosensor for Visualizing the Tension across Paxillin in Living Cells upon Shear Stress. Analysis and Sensing, 2(1), Article e202100033. https://doi.org/10.1002/anse.202100033

JYU-tekijät tai -toimittajat

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Shao, Shuai; Deng, Sha; Jiang, Qingyun; Zhang, Hangyu; Zhang, Zhengyao; Li, Na; Cong, Fengyu; Tiihonen, Timo; Liu, Bo

Lehti tai sarja: Analysis and Sensing

eISSN: 2629-2742

Julkaisuvuosi: 2022

Ilmestymispäivä: 13.10.2021

Volyymi: 2

Lehden numero: 1

Artikkelinumero: e202100033

Kustantaja: Wiley

Julkaisumaa: Saksa

Julkaisun kieli: englanti

DOI: https://doi.org/10.1002/anse.202100033

Julkaisun avoin saatavuus: Ei avoin

Julkaisukanavan avoin saatavuus:

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/78225


Paxillin is a potential participant in the direct intracellular force transmission which is considered as the foundation of cells sensing and responding to extracellular environment. However, the detection of tension across paxillin has not been achieved due to lacking microsized tools. Herein, a paxillin tension sensor (PaxTs) based on Fluorescence Resonance Energy Transfer (FRET) technique was constructed. PaxTs can be expressed and assembled to FA sites spontaneously to visualize the tension across paxillin with FRET efficiency of ~62.4% in living cells. The tension across paxillin was found to decrease upon shear stress, in which the membrane fluidity and contractility of actin acted as cushions. It is observed that paxillin participates in the pathway of cell membrane-cytoskeleton-FAs for force transmission upon mechanical force in real time visualization, which provides a promising new method to investigate the direct intracellular force transmission in biology and technology.

YSO-asiasanat: biosensorit; solufysiologia; soluviestintä; proteiinit; mekaniikka

Vapaat asiasanat: biosensors; focal adhesions; FRET; paxillin; shear stress

Liittyvät organisaatiot

OKM-raportointi: Kyllä

Raportointivuosi: 2022

Viimeisin päivitys 2022-20-09 klo 14:47