A1 Journal article (refereed)
A DNA‐Encoded FRET Biosensor for Visualizing the Tension across Paxillin in Living Cells upon Shear Stress (2022)
Shao, S., Deng, S., Jiang, Q., Zhang, H., Zhang, Z., Li, N., Cong, F., Tiihonen, T., & Liu, B. (2022). A DNA‐Encoded FRET Biosensor for Visualizing the Tension across Paxillin in Living Cells upon Shear Stress. Analysis and Sensing, 2(1), Article e202100033. https://doi.org/10.1002/anse.202100033
JYU authors or editors
Publication details
All authors or editors: Shao, Shuai; Deng, Sha; Jiang, Qingyun; Zhang, Hangyu; Zhang, Zhengyao; Li, Na; Cong, Fengyu; Tiihonen, Timo; Liu, Bo
Journal or series: Analysis and Sensing
eISSN: 2629-2742
Publication year: 2022
Publication date: 13/10/2021
Volume: 2
Issue number: 1
Article number: e202100033
Publisher: Wiley
Publication country: Germany
Publication language: English
DOI: https://doi.org/10.1002/anse.202100033
Publication open access: Not open
Publication channel open access:
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/78225
Abstract
Paxillin is a potential participant in the direct intracellular force transmission which is considered as the foundation of cells sensing and responding to extracellular environment. However, the detection of tension across paxillin has not been achieved due to lacking microsized tools. Herein, a paxillin tension sensor (PaxTs) based on Fluorescence Resonance Energy Transfer (FRET) technique was constructed. PaxTs can be expressed and assembled to FA sites spontaneously to visualize the tension across paxillin with FRET efficiency of ~62.4% in living cells. The tension across paxillin was found to decrease upon shear stress, in which the membrane fluidity and contractility of actin acted as cushions. It is observed that paxillin participates in the pathway of cell membrane-cytoskeleton-FAs for force transmission upon mechanical force in real time visualization, which provides a promising new method to investigate the direct intracellular force transmission in biology and technology.
Keywords: biosensors; cell physiology; cell signaling; proteins; mechanics
Contributing organizations
Ministry reporting: Yes
Reporting Year: 2022
