A1 Journal article (refereed)
Potent Inhibitor of Human Trypsins from the Aeruginosin Family of Natural Products (2021)
Ahmed, M. N., Wahlsten, M., Jokela, J., Nees, M., Stenman, U.-H., Alvarenga, D. O., Strandin, T., Sivonen, K., Poso, A., Permi, P., Metsä-Ketelä, M., Koistinen, H., & Fewer, D. P. (2021). Potent Inhibitor of Human Trypsins from the Aeruginosin Family of Natural Products. ACS Chemical Biology, 16(11), 2537-2546. https://doi.org/10.1021/acschembio.1c00611
JYU authors or editors
Publication details
All authors or editors: Ahmed, Muhammad N.; Wahlsten, Matti; Jokela, Jouni; Nees, Matthias; Stenman, Ulf-Håkan; Alvarenga, Danillo O.; Strandin, Tomas; Sivonen, Kaarina; Poso, Antti; Permi, Perttu; et al.
Journal or series: ACS Chemical Biology
ISSN: 1554-8929
eISSN: 1554-8937
Publication year: 2021
Publication date: 18/10/2021
Volume: 16
Issue number: 11
Pages range: 2537-2546
Publisher: American Chemical Society (ACS)
Publication country: United States
Publication language: English
DOI: https://doi.org/10.1021/acschembio.1c00611
Publication open access: Openly available
Publication channel open access: Partially open access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/78270
Abstract
Serine proteases regulate many physiological processes and play a key role in a variety of cancers. Aeruginosins are a family of natural products produced by cyanobacteria that exhibit pronounced structural diversity and potent serine protease inhibition. Here, we sequenced the complete genome of Nodularia sphaerocarpa UHCC 0038 and identified the 43.7 kb suomilide biosynthetic gene cluster. Bioinformatic analysis demonstrated that suomilide belongs to the aeruginosin family of natural products. We identified 103 complete aeruginosin biosynthetic gene clusters from 12 cyanobacterial genera and showed that they encode an unexpected chemical diversity. Surprisingly, purified suomilide inhibited human trypsin-2 and -3, with IC50 values of 4.7 and 11.5 nM, respectively, while trypsin-1 was inhibited with an IC50 of 104 nM. Molecular dynamics simulations suggested that suomilide has a long residence time when bound to trypsins. This was confirmed experimentally for trypsin-1 and -3 (residence times of 1.5 and 57 min, respectively). Suomilide also inhibited the invasion of aggressive and metastatic PC-3M prostate cancer cells without affecting cell proliferation. The potent inhibition of trypsin-3, together with a long residence time and the ability to inhibit prostate cancer cell invasion, makes suomilide an attractive drug lead for targeting cancers that overexpress trypsin-3. These results substantially broaden the genetic and chemical diversity of the aeruginosin family and suggest that aeruginosins may be a source of selective inhibitors of human serine proteases.
Keywords: natural products; proteins; proteomics; inhibitors; cancer cells; cancerous diseases; biochemistry; bioinformatics
Free keywords: proteases; serine proteases; Aeruginosins
Contributing organizations
Ministry reporting: Yes
Reporting Year: 2021
JUFO rating: 2