A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Microbial communities in full-scale woodchip bioreactors treating aquaculture effluents (2022)

Aalto, S. L., Suurnäkki, S., von Ahnen, M., Tiirola, M., & Bovbjerg Pedersen, P. (2022). Microbial communities in full-scale woodchip bioreactors treating aquaculture effluents. Journal of Environmental Management, 301, Article 113852. https://doi.org/10.1016/j.jenvman.2021.113852

JYU-tekijät tai -toimittajat

Aalto, Sanni
Suurnäkki, Suvi
Tiirola, Marja

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Aalto, Sanni L.; Suurnäkki, Suvi; von Ahnen, Mathis; Tiirola, Marja; Bovbjerg Pedersen, Per

Lehti tai sarja: Journal of Environmental Management

ISSN: 0301-4797

eISSN: 1095-8630

Julkaisuvuosi: 2022

Volyymi: 301

Artikkelinumero: 113852

Kustantaja: Elsevier Ltd.

Julkaisumaa: Britannia

Julkaisun kieli: englanti

DOI: https://doi.org/10.1016/j.jenvman.2021.113852

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/78357

Tiivistelmä

Woodchip bioreactors are being successfully applied to remove nitrate from commercial land-based recirculating aquaculture system (RAS) effluents. In order to understand and optimize the overall function of these bioreactors, knowledge on the microbial communities, especially on the microbes with potential for production or mitigation of harmful substances (e.g. hydrogen sulfide; H2S) is needed. In this study, we quantified and characterized bacterial and fungal communities, including potential H2S producers and consumers, using qPCR and high throughput sequencing of 16S rRNA gene. We took water samples from bioreactors and their inlet and outlet, and sampled biofilms growing on woodchips and on the outlet of the three full-scale woodchip bioreactors treating effluents of three individual RAS. We found that bioreactors hosted a high biomass of both bacteria and fungi. Although the composition of microbial communities of the inlet varied between the bioreactors, the conditions in the bioreactors selected for the same core microbial taxa. The H2S producing sulfate reducing bacteria (SRB) were mainly found in the nitrate-limited outlets of the bioreactors, the main groups being deltaproteobacterial Desulfobulbus and Desulfovibrio. The abundance of H2S consuming sulfate oxidizing bacteria (SOB) was 5–10 times higher than that of SRB, and SOB communities were dominated by Arcobacter and other genera from phylum Epsilonbacteraeota, which are also capable of autotrophic denitrification. Indeed, the relative abundance of potential autotrophic denitrifiers of all denitrifier sequences was even 54% in outlet water samples and 56% in the outlet biofilm samples. Altogether, our results show that the highly abundant bacterial and fungal communities in woodchip bioreactors are shaped through the conditions prevailing within the bioreactor, indicating that the bioreactors with similar design and operational settings should provide similar function even when conditions in the preceding RAS would differ. Furthermore, autotrophic denitrifiers can have a significant role in woodchip biofilters, consuming potentially produced H2S and removing nitrate, lengthening the operational age and thus further improving the overall environmental benefit of these bioreactors.

YSO-asiasanat: vesiviljely (kalatalous); jäteveden käsittely; denitrifikaatio; bioreaktorit; hake; mikrobisto; sienet; sulfidit

Vapaat asiasanat: aquaculture; denitrification; fungi; microbiome; sulfate reduction

Liittyvät organisaatiot

Hankkeet, joissa julkaisu on tehty

OKM-raportointi: Kyllä

Raportointivuosi: 2022

JUFO-taso: 1