A3 Book section, Chapters in research books
Construction of Metatranscriptomic Libraries for 5′ End Sequencing of rRNAs for Microbiome Research (2021)

Tiirola, M., & Mäki, A. (2021). Construction of Metatranscriptomic Libraries for 5′ End Sequencing of rRNAs for Microbiome Research. In A. Navid (Ed.), Microbial Systems Biology : Methods and Protocols (pp. 137-146). Humana Press. Methods in Molecular Biology, 2349. https://doi.org/10.1007/978-1-0716-1585-0_7

JYU authors or editors

Publication details

All authors or editors: Tiirola, Marja; Mäki, Anita

Parent publication: Microbial Systems Biology : Methods and Protocols

Parent publication editors: Navid, Ali

ISBN: 978-1-0716-1584-3

eISBN: 978-1-0716-1585-0

Journal or series: Methods in Molecular Biology

ISSN: 1064-3745

eISSN: 1940-6029

Publication year: 2021

Publication date: 31/10/2021

Number in series: 2349

Pages range: 137-146

Number of pages in the book: 385

Publisher: Humana Press

Place of Publication: New York

Publication country: United States

Publication language: English

DOI: https://doi.org/10.1007/978-1-0716-1585-0_7

Publication open access: Not open

Publication channel open access: Channel is not openly available

Abstract

Metatranscriptomic sequencing enables studying community-wide gene expression profiles of microbial samples and getting functional insight on their up- or downregulated pathways. However, shotgun sequencing is not the most efficient way to study expression of ribosomal RNA genes or to compare lot of samples in experimental setups. Here we describe an efficient primer-independent method for processing and barcoding libraries for directional sequencing of the 5′ end region of the RNA. When applying size selection of the original RNA, the method forms an optimal solution for the simultaneous analysis of bacterial, archaeal, and eukaryotic rRNA diversity.

Keywords: microbiome; research methods; sequencing; RNA; transcriptome

Free keywords: RNA; metatranscriptomics; microbiomes; high-throughput; sequencing; NGS; SSU; rRNA

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Related projects

Ministry reporting: Yes

Reporting Year: 2021

JUFO rating: 1