A1 Journal article (refereed)
Functional roles of the membrane-associated AAV protein MAAP (2021)
Galibert, L., Hyvönen, A., Eriksson, R. A. E., Mattola, S., Aho, V., Salminen, S., Albers, J. D., Peltola, S. K., Weman, S., Nieminen, T., Ylä-Herttuala, S., Lesch, H. P., Vihinen-Ranta, M., & Airenne, K. J. (2021). Functional roles of the membrane-associated AAV protein MAAP. Scientific Reports, 11, Article 21698. https://doi.org/10.1038/s41598-021-01220-7
JYU authors or editors
Publication details
All authors or editors: Galibert, Lionel; Hyvönen, Amira; Eriksson, Reetta A. E.; Mattola, Salla; Aho, Vesa; Salminen, Sami; Albers, Justin D.; Peltola, Sanna K.; Weman, Saija; Nieminen, Tiina; et al.
Journal or series: Scientific Reports
eISSN: 2045-2322
Publication year: 2021
Publication date: 04/11/2021
Volume: 11
Article number: 21698
Publisher: Nature Publishing Group
Publication country: United Kingdom
Publication language: English
DOI: https://doi.org/10.1038/s41598-021-01220-7
Publication open access: Openly available
Publication channel open access: Open Access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/78516
Abstract
nucleus and to the nuclear envelope by co-transfecting with plasmids encoding the wild-type AAV (wt-AAV) genome and adenovirus (Ad) helper genes. While keeping VP1/2 protein sequence identical, both inactivation and truncation of MAAP translation afected the emergence and intracellular distribution of the AAV capsid proteins. We have demonstrated that MAAP facilitates AAV replication and has a role in controlling Ad infection. Additionally, we were able to improve virus production and capsid integrity through a C-terminal truncation of MAAP while other modifcations led to increased packaging of contaminating, non-viral DNA. Our results show that MAAP plays a signifcant role in AAV infection, with profound implications for the production of therapeutic AAV vectors.
Keywords: parvoviruses; infections; proteins; capsid
Contributing organizations
Related projects
- Way out through chromatin: nuclear exit of herpesvirus mRNA and capsids
- Vihinen-Ranta, Maija
- Research Council of Finland
Ministry reporting: Yes
VIRTA submission year: 2021
JUFO rating: 1