A1 Journal article (refereed)
Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane (2021)
Aho, V., Salminen, S., Mattola, S., Gupta, A., Flomm, F., Sodeik, B., Bosse, J. B., & Vihinen-Ranta, M. (2021). Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane. PLoS Pathogens, 17(12), Article e1010132. https://doi.org/10.1371/journal.ppat.1010132
JYU authors or editors
Publication details
All authors or editors: Aho, Vesa; Salminen, Sami; Mattola, Salla; Gupta, Alka; Flomm, Felix; Sodeik, Beate; Bosse, Jens B.; Vihinen-Ranta, Maija
Journal or series: PLoS Pathogens
ISSN: 1553-7366
eISSN: 1553-7374
Publication year: 2021
Publication date: 15/12/2021
Volume: 17
Issue number: 12
Article number: e1010132
Publisher: Public Library of Science (PLoS)
Publication country: United States
Publication language: English
DOI: https://doi.org/10.1371/journal.ppat.1010132
Publication open access: Openly available
Publication channel open access: Open Access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/79069
Abstract
Herpes simplex virus capsids are assembled and packaged in the nucleus and move by diffusion through the nucleoplasm to the nuclear envelope for egress. Analyzing their motion provides conclusions not only on capsid transport but also on the properties of the nuclear environment during infection. We utilized live-cell imaging and single-particle tracking to characterize capsid motion relative to the host chromatin. The data indicate that as the chromatin was marginalized toward the nuclear envelope it presented a restrictive barrier to the capsids. However, later in infection this barrier became more permissive and the probability of capsids to enter the chromatin increased. Thus, although chromatin marginalization initially restricted capsid transport to the nuclear envelope, a structural reorganization of the chromatin counteracted that to promote capsid transport later. Analyses of capsid motion revealed that it was subdiffusive, and that the diffusion coefficients were lower in the chromatin than in regions lacking chromatin. In addition, the diffusion coefficient in both regions increased during infection. Throughout the infection, the capsids were never enriched at the nuclear envelope, which suggests that instead of nuclear export the transport through the chromatin is the rate-limiting step for the nuclear egress of capsids. This provides motivation for further studies by validating the importance of intranuclear transport to the life cycle of HSV-1.
Keywords: herpesviruses; herpes simplex virus; infections; capsid; diffusion (physical phenomena)
Contributing organizations
Related projects
- Way out through chromatin: nuclear exit of herpesvirus mRNA and capsids
- Vihinen-Ranta, Maija
- Research Council of Finland
- Compact Cell-Imaging Device to provide insight into the cellular origins of diseases and to aid in the development of novel therapeutics
- Vihinen-Ranta, Maija
- European Commission
Ministry reporting: Yes
VIRTA submission year: 2021
JUFO rating: 2