A1 Journal article (refereed)
Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold (2022)

Samanta, S., Ylä-Outinen, L., Rangasami, V. K., Narkilahti, S., & Oommen, O. P. (2022). Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold. Acta Biomaterialia, 140, 314-323. https://doi.org/10.1016/j.actbio.2021.12.010

JYU authors or editors

Publication details

All authors or editors: Samanta, Sumanta; Ylä-Outinen, Laura; Rangasami, Vignesh Kumar; Narkilahti, Susanna; Oommen, Oommen P.

Journal or series: Acta Biomaterialia

ISSN: 1742-7061

eISSN: 1878-7568

Publication year: 2022

Volume: 140

Pages range: 314-323

Publisher: Elsevier

Publication country: Netherlands

Publication language: English

DOI: https://doi.org/10.1016/j.actbio.2021.12.010

Publication open access: Openly available

Publication channel open access: Partially open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/79174

Additional information: Corrigendum: Acta Biomaterialia, Volume 147, 15 July 2022, Page 439. https://doi.org/10.1016/j.actbio.2022.05.025

Abstract

Human pluripotent stem cells (hPSC) derived neurons are emerging as a powerful tool for studying neurobiology, disease pathology, and modeling. Due to the lack of platforms available for housing and growing hPSC-derived neurons, a pressing need exists to tailor a brain-mimetic 3D scaffold that recapitulates tissue composition and favourably regulates neuronal network formation. Despite the progress in engineering biomimetic scaffolds, an ideal brain-mimetic scaffold is still elusive. We bioengineered a physiologically relevant 3D scaffold by integrating brain-like extracellular matrix (ECM) components and chemical cues. Culturing hPSCs-neurons in hyaluronic acid (HA) gels and HA-chondroitin sulfate (HA-CS) composite gels showed that the CS component prevails as the predominant factor for the growth of neuronal cells, albeit to modest efficacy. Covalent grafting of dopamine (DA) moieties to the HA-CS gel (HADA-CS) enhanced the scaffold stability and stimulated the gel's remodeling properties by entrapping cell-secreted laminin, and binding brain-derived neurotrophic factor (BDNF). Neurons cultured in the scaffold expressed Col1, Col11, and ITGB4; important for cell adhesion and cell-ECM signaling. Thus, the HA-CS scaffold with integrated chemical cues (DA) supported neuronal growth and network formation. This scaffold offers a valuable tool for tissue engineering and disease modeling and helps in bridging the gap between animal models and human diseases by providing biomimetic neurophysiology.

Keywords: tissue culture; neurons; neural networks (biology); induced pluripotent stem cells; biomimetic materials; hyaluronan; dopamine

Free keywords: neuronal network; human pluripotent stem cells; hyaluronic acid; chondroitin sulfate; dopamine; brain-mimetic hydrogel scaffold

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Ministry reporting: Yes

VIRTA submission year: 2022

JUFO rating: 2