A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold (2022)


Samanta, S., Ylä-Outinen, L., Rangasami, V. K., Narkilahti, S., & Oommen, O. P. (2022). Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold. Acta Biomaterialia, 140, 314-323. https://doi.org/10.1016/j.actbio.2021.12.010


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Samanta, Sumanta; Ylä-Outinen, Laura; Rangasami, Vignesh Kumar; Narkilahti, Susanna; Oommen, Oommen P.

Lehti tai sarja: Acta Biomaterialia

ISSN: 1742-7061

eISSN: 1878-7568

Julkaisuvuosi: 2022

Volyymi: 140

Artikkelin sivunumerot: 314-323

Kustantaja: Elsevier

Julkaisumaa: Alankomaat

Julkaisun kieli: englanti

DOI: https://doi.org/10.1016/j.actbio.2021.12.010

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/79174


Tiivistelmä

Human pluripotent stem cells (hPSC) derived neurons are emerging as a powerful tool for studying neurobiology, disease pathology, and modeling. Due to the lack of platforms available for housing and growing hPSC-derived neurons, a pressing need exists to tailor a brain-mimetic 3D scaffold that recapitulates tissue composition and favourably regulates neuronal network formation. Despite the progress in engineering biomimetic scaffolds, an ideal brain-mimetic scaffold is still elusive. We bioengineered a physiologically relevant 3D scaffold by integrating brain-like extracellular matrix (ECM) components and chemical cues. Culturing hPSCs-neurons in hyaluronic acid (HA) gels and HA-chondroitin sulfate (HA-CS) composite gels showed that the CS component prevails as the predominant factor for the growth of neuronal cells, albeit to modest efficacy. Covalent grafting of dopamine (DA) moieties to the HA-CS gel (HADA-CS) enhanced the scaffold stability and stimulated the gel's remodeling properties by entrapping cell-secreted laminin, and binding brain-derived neurotrophic factor (BDNF). Neurons cultured in the scaffold expressed Col1, Col11, and ITGB4; important for cell adhesion and cell-ECM signaling. Thus, the HA-CS scaffold with integrated chemical cues (DA) supported neuronal growth and network formation. This scaffold offers a valuable tool for tissue engineering and disease modeling and helps in bridging the gap between animal models and human diseases by providing biomimetic neurophysiology.


YSO-asiasanat: kudosviljely; hermosolut; hermoverkot (biologia); indusoidut monikykyiset kantasolut; biomimeettiset materiaalit; hyaluronaani; dopamiini

Vapaat asiasanat: neuronal network; human pluripotent stem cells; hyaluronic acid; chondroitin sulfate; dopamine; brain-mimetic hydrogel scaffold


Liittyvät organisaatiot


Hankkeet, joissa julkaisu on tehty


OKM-raportointi: Kyllä

Alustava JUFO-taso: 2


Viimeisin päivitys 2022-17-06 klo 10:47