A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold (2022)


Samanta, S., Ylä-Outinen, L., Rangasami, V. K., Narkilahti, S., & Oommen, O. P. (2022). Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold. Acta Biomaterialia, 140, 314-323. https://doi.org/10.1016/j.actbio.2021.12.010


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajatSamanta, Sumanta; Ylä-Outinen, Laura; Rangasami, Vignesh Kumar; Narkilahti, Susanna; Oommen, Oommen P.

Lehti tai sarjaActa Biomaterialia

ISSN1742-7061

eISSN1878-7568

Julkaisuvuosi2022

Volyymi140

Artikkelin sivunumerot314-323

KustantajaElsevier

JulkaisumaaAlankomaat

Julkaisun kielienglanti

DOIhttps://doi.org/10.1016/j.actbio.2021.12.010

Julkaisun avoin saatavuusAvoimesti saatavilla

Julkaisukanavan avoin saatavuusOsittain avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX)https://jyx.jyu.fi/handle/123456789/79174

LisätietojaCorrigendum: Acta Biomaterialia, Volume 147, 15 July 2022, Page 439. https://doi.org/10.1016/j.actbio.2022.05.025


Tiivistelmä

Human pluripotent stem cells (hPSC) derived neurons are emerging as a powerful tool for studying neurobiology, disease pathology, and modeling. Due to the lack of platforms available for housing and growing hPSC-derived neurons, a pressing need exists to tailor a brain-mimetic 3D scaffold that recapitulates tissue composition and favourably regulates neuronal network formation. Despite the progress in engineering biomimetic scaffolds, an ideal brain-mimetic scaffold is still elusive. We bioengineered a physiologically relevant 3D scaffold by integrating brain-like extracellular matrix (ECM) components and chemical cues. Culturing hPSCs-neurons in hyaluronic acid (HA) gels and HA-chondroitin sulfate (HA-CS) composite gels showed that the CS component prevails as the predominant factor for the growth of neuronal cells, albeit to modest efficacy. Covalent grafting of dopamine (DA) moieties to the HA-CS gel (HADA-CS) enhanced the scaffold stability and stimulated the gel's remodeling properties by entrapping cell-secreted laminin, and binding brain-derived neurotrophic factor (BDNF). Neurons cultured in the scaffold expressed Col1, Col11, and ITGB4; important for cell adhesion and cell-ECM signaling. Thus, the HA-CS scaffold with integrated chemical cues (DA) supported neuronal growth and network formation. This scaffold offers a valuable tool for tissue engineering and disease modeling and helps in bridging the gap between animal models and human diseases by providing biomimetic neurophysiology.


YSO-asiasanatkudosviljelyhermosoluthermoverkot (biologia)indusoidut monikykyiset kantasolutbiomimeettiset materiaalithyaluronaanidopamiini

Vapaat asiasanatneuronal network; human pluripotent stem cells; hyaluronic acid; chondroitin sulfate; dopamine; brain-mimetic hydrogel scaffold


Liittyvät organisaatiot


Hankkeet, joissa julkaisu on tehty


OKM-raportointiKyllä

Raportointivuosi2022

JUFO-taso2


Viimeisin päivitys 2024-26-03 klo 09:20