A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold (2022)
Samanta, S., Ylä-Outinen, L., Rangasami, V. K., Narkilahti, S., & Oommen, O. P. (2022). Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold. Acta Biomaterialia, 140, 314-323. https://doi.org/10.1016/j.actbio.2021.12.010
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Samanta, Sumanta; Ylä-Outinen, Laura; Rangasami, Vignesh Kumar; Narkilahti, Susanna; Oommen, Oommen P.
Lehti tai sarja: Acta Biomaterialia
ISSN: 1742-7061
eISSN: 1878-7568
Julkaisuvuosi: 2022
Volyymi: 140
Artikkelin sivunumerot: 314-323
Kustantaja: Elsevier
Julkaisumaa: Alankomaat
Julkaisun kieli: englanti
DOI: https://doi.org/10.1016/j.actbio.2021.12.010
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/79174
Lisätietoja: Corrigendum: Acta Biomaterialia, Volume 147, 15 July 2022, Page 439. https://doi.org/10.1016/j.actbio.2022.05.025
Tiivistelmä
Human pluripotent stem cells (hPSC) derived neurons are emerging as a powerful tool for studying neurobiology, disease pathology, and modeling. Due to the lack of platforms available for housing and growing hPSC-derived neurons, a pressing need exists to tailor a brain-mimetic 3D scaffold that recapitulates tissue composition and favourably regulates neuronal network formation. Despite the progress in engineering biomimetic scaffolds, an ideal brain-mimetic scaffold is still elusive. We bioengineered a physiologically relevant 3D scaffold by integrating brain-like extracellular matrix (ECM) components and chemical cues. Culturing hPSCs-neurons in hyaluronic acid (HA) gels and HA-chondroitin sulfate (HA-CS) composite gels showed that the CS component prevails as the predominant factor for the growth of neuronal cells, albeit to modest efficacy. Covalent grafting of dopamine (DA) moieties to the HA-CS gel (HADA-CS) enhanced the scaffold stability and stimulated the gel's remodeling properties by entrapping cell-secreted laminin, and binding brain-derived neurotrophic factor (BDNF). Neurons cultured in the scaffold expressed Col1, Col11, and ITGB4; important for cell adhesion and cell-ECM signaling. Thus, the HA-CS scaffold with integrated chemical cues (DA) supported neuronal growth and network formation. This scaffold offers a valuable tool for tissue engineering and disease modeling and helps in bridging the gap between animal models and human diseases by providing biomimetic neurophysiology.
YSO-asiasanat: kudosviljely; hermosolut; hermoverkot (biologia); indusoidut monikykyiset kantasolut; biomimeettiset materiaalit; hyaluronaani; dopamiini
Vapaat asiasanat: neuronal network; human pluripotent stem cells; hyaluronic acid; chondroitin sulfate; dopamine; brain-mimetic hydrogel scaffold
Liittyvät organisaatiot
Hankkeet, joissa julkaisu on tehty
- Yliopistojen profiloitumisen vahvistaminen kilpaillulla rahoituksella. Profilointitoimet JYU:ssä, 2. kierros
- Hämäläinen, Keijo
- Suomen Akatemia
OKM-raportointi: Kyllä
Raportointivuosi: 2022
JUFO-taso: 2