A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
How to reach optimal estimates of confidence intervals in microscopic counting of phytoplankton? (2021)
Salonen, K., Salmi, P., & Keskitalo, J. (2021). How to reach optimal estimates of confidence intervals in microscopic counting of phytoplankton?. Journal of Plankton Research, 43(6), 846-852. https://doi.org/10.1093/plankt/fbab062
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Salonen, Kalevi; Salmi, Pauliina; Keskitalo, Jorma
Lehti tai sarja: Journal of Plankton Research
ISSN: 0142-7873
eISSN: 1464-3774
Julkaisuvuosi: 2021
Ilmestymispäivä: 04.10.2021
Volyymi: 43
Lehden numero: 6
Artikkelin sivunumerot: 846-852
Kustantaja: Oxford University Press (OUP)
Julkaisumaa: Britannia
Julkaisun kieli: englanti
DOI: https://doi.org/10.1093/plankt/fbab062
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/79233
Tiivistelmä
Present practices in the microscopic counting of phytoplankton to estimate the reliability of results rely on the assumption of a random distribution of taxa in sample preparations. In contrast to that and in agreement with the literature, we show that aggregated distribution is common and can lead to over-optimistic confidence intervals, if estimated according to the shortcut procedure of Lund et al. based on the number of counted cells. We found a good linear correlation between the distribution independent confidence intervals for medians and those for parametric statistics so that 95% confidence intervals can be approximated by using a correction factor of 1.4. Instead, the recommendation to estimate confidence intervals from the total number of counted cells according to Lund et al. should be categorically rejected. We further propose the adoption of real-time confidence intervals during microscopic counting as the criterion to define how long counting should be continued. Then each sample can be counted in its individual way to reach the necessary reliability independent of highly different samples. Such a dynamic counting strategy would be the most significant development in the quality control of phytoplankton counting since the early pioneers established the present counting practices in the late 1950s.
YSO-asiasanat: plankton; mikrolevät; laskeminen; mikroskopia; estimointi; tilastomenetelmät; luottamustasot
Liittyvät organisaatiot
OKM-raportointi: Kyllä
Raportointivuosi: 2021
JUFO-taso: 1
