A1 Journal article (refereed)
Exposure to retene, fluoranthene, and their binary mixture causes distinct transcriptomic and apical outcomes in rainbow trout (Oncorhynchus mykiss) yolk sac alevins (2022)
Eriksson, A. N., Rigaud, C., Krasnov, A., Wincent, E., & Vehniäinen, E.-R. (2022). Exposure to retene, fluoranthene, and their binary mixture causes distinct transcriptomic and apical outcomes in rainbow trout (Oncorhynchus mykiss) yolk sac alevins. Aquatic Toxicology, 244, Article 106083. https://doi.org/10.1016/j.aquatox.2022.106083
JYU authors or editors
Publication details
All authors or editors: Eriksson, Andreas N.M.; Rigaud, Cyril; Krasnov, Aleksei; Wincent, Emma; Vehniäinen, Eeva-Riikka
Journal or series: Aquatic Toxicology
ISSN: 0166-445X
eISSN: 1879-1514
Publication year: 2022
Volume: 244
Article number: 106083
Publisher: Elsevier
Publication country: Netherlands
Publication language: English
DOI: https://doi.org/10.1016/j.aquatox.2022.106083
Publication open access: Openly available
Publication channel open access: Partially open access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/79608
Abstract
Polycyclic aromatic hydrocarbons (PAHs) are widely spread environmental contaminants which affect developing organisms. It is known that improper activation of the aryl hydrocarbon receptor (AhR) by some PAHs contributes to toxicity, while other PAHs can disrupt cellular membrane function. The exact downstream mechanisms of AhR activation remain unresolved, especially with regard to cardiotoxicity. By exposing newly hatched rainbow trout alevins (Oncorhynchus mykiss) semi-statically to retene (32 µg l−1; AhR agonist), fluoranthene (50 µg l−1; weak AhR agonist and CYP1a inhibitor) and their binary mixture for 1, 3, 7 and 14 days, we aimed to uncover novel mechanisms of cardiotoxicity using a targeted microarray approach. At the end of the exposure, standard length, yolk area, blue sac disease (BSD) index and PAH body burden were measured, while the hearts were prepared for microarray analysis. Each exposure produced a unique toxicity profile. We observed that retene and the mixture, but not fluoranthene, significantly reduced growth by Day 14 compared to the control, while exposure to the mixture increased the BSD-index significantly from Day 3 onward. Body burden profiles were PAH-specific and correlated well with the exposure-specific upregulations of genes encoding for phase I and II enzymes. Exposure to the mixture over-represented pathways related to growth, amino acid and xenobiotic metabolism and oxidative stress responses. Alevins exposed to the individual PAHs displayed over-represented pathways involved in receptor signaling: retene downregulated genes with a role in G-protein signaling, while fluoranthene upregulated those involved in GABA signaling. Furthermore, exposure to retene and fluoranthene altered the expression of genes encoding for proteins involved in calcium- and potassium ion channels, which suggests affected heart structure and function. This study provides deeper understanding of the complexity of PAH toxicity and the necessity of investigating PAHs as mixtures and not as individual components.
Keywords: environmental toxins; PAH compounds; mixtures; embryogenesis; transcriptome; rainbow trout; ecotoxicology
Free keywords: Mixture; Retene; Fluoranthene; PAH; Oncorhynchus mykiss; Early life development
Contributing organizations
Related projects
- Integrated OMICS and imaging for a better understanding of ecotoxicological mechanisms - PAH developmental toxicity as an example
- Vehniäinen, Eeva-Riikka
- Research Council of Finland
- Integrated OMICS and imaging for a better understanding of ecotoxicological mechanisms
- Vehniäinen, Eeva-Riikka
- Research Council of Finland
- Integrated OMICS and imaging for a better understanding of ecotoxicological mechanisms - PAH developmental toxicity as an example (research costs)
- Vehniäinen, Eeva-Riikka
- Research Council of Finland
Ministry reporting: Yes
Reporting Year: 2022
JUFO rating: 1