A1 Journal article (refereed)
Repeated exposure of fluazinam fungicides affects gene expression profiles yet carries no costs on a nontarget pest (2022)

Saifullah, S., Margus, A., Kankare, M., & Lindström, L. (2022). Repeated exposure of fluazinam fungicides affects gene expression profiles yet carries no costs on a nontarget pest. Insect Science, 29(5), 1373-1386. https://doi.org/10.1111/1744-7917.13013

JYU authors or editors

Publication details

All authors or editors: Saifullah, Shahed; Margus, Aigi; Kankare, Maaria; Lindström, Leena

Journal or series: Insect Science

ISSN: 1672-9609

eISSN: 1744-7917

Publication year: 2022

Publication date: 10/02/2022

Volume: 29

Issue number: 5

Pages range: 1373-1386

Publisher: Wiley-Blackwell

Publication country: Australia

Publication language: English

DOI: https://doi.org/10.1111/1744-7917.13013

Publication open access: Openly available

Publication channel open access: Partially open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/79847

Abstract

Fungicides are used to control pathogenic fungi of crop species, but they have also been shown to alter behavioral, life history and fitness related traits of non-target insects. Here, we tested the fungicide effects on feeding behavior, survival and physiology of the non-target pest insect, the Colorado potato beetle (Leptinotarsa decemlineata). Feeding behavior was studied by a choice test of adult beetles, which were allowed to choose between a control and a fungicide (fluazinam) treated potato leaf. Larval survival was recorded after 24-hour and 72-hour exposure to control and fungicide-treated leaves with two different concentrations. The adults did not show fungicide avoidance behavior. Similarly, survival of the larvae was not affected by the exposure to fungicides. Finally, to understand the effects of fungicides at the physiological level (gene expression), we tested whether the larval exposure to fungicide alter the expression of five metabolic pathway and stress associated genes. Highest concentration and 72-hour exposure caused upregulation of one cytochrome P450 (CYP9Z14v2) and one insecticide resistance gene (Ldace1), whereas metabolic detoxification gene (Ugt1) was downregulated. 24-hour exposure to highest concentration caused downregulation of another common detoxification gene (Gs), while both exposure times to lowest concentration caused upregulation of the Hsp70 stress tolerance gene. Despite these overall effects, there was a considerable amount of variation among different families in the gene expression levels. Even though the behavioral effects of the fungicide treatments were minor, the expression level differences of the studied genes indicate changes on the metabolic detoxifications and stress-related pathways.

Keywords: fungicides; exposure; gene expression; metabolism; animal behaviour; insect pests; Colorado potato beetle

Free keywords: behavior; fluazinam; gene expression; Leptinotarsa decemlineata; metabolic detoxification; non-target animal

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Ministry reporting: Yes

VIRTA submission year: 2022

JUFO rating: 1