A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Gap junctions and connexin hemichannels both contribute to the electrical properties of retinal pigment epithelium (2022)


Fadjukov, J., Wienbar, S., Hakanen, S., Aho, V., Vihinen-Ranta, M., Ihalainen, T. O., Schwartz, G. W., & Nymark, S. (2022). Gap junctions and connexin hemichannels both contribute to the electrical properties of retinal pigment epithelium. Journal of General Physiology, 154(4), Article e202112916. https://doi.org/10.1085/jgp.202112916


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajatFadjukov, Julia; Wienbar, Sophia; Hakanen, Satu; Aho, Vesa; Vihinen-Ranta, Maija; Ihalainen, Teemu O.; Schwartz, Gregory W.; Nymark, Soile

Lehti tai sarjaJournal of General Physiology

ISSN0022-1295

eISSN1540-7748

Julkaisuvuosi2022

Ilmestymispäivä11.03.2022

Volyymi154

Lehden numero4

Artikkelinumeroe202112916

KustantajaRockefeller University Press

JulkaisumaaYhdysvallat (USA)

Julkaisun kielienglanti

DOIhttps://doi.org/10.1085/jgp.202112916

Julkaisun avoin saatavuusAvoimesti saatavilla

Julkaisukanavan avoin saatavuusOsittain avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX)https://jyx.jyu.fi/handle/123456789/83205


Tiivistelmä

Gap junctions are intercellular channels that permit the transfer of ions and small molecules between adjacent cells. These cellular junctions are particularly dense in the retinal pigment epithelium (RPE), and their contribution to many retinal diseases has been recognized. While gap junctions have been implicated in several aspects of RPE physiology, their role in shaping the electrical properties of these cells has not been characterized in mammals. The role of gap junctions in the electrical properties of the RPE is particularly important considering the growing appreciation of RPE as excitable cells containing various voltage-gated channels. We used a whole-cell patch clamp to measure the electrical characteristics and connectivity between RPE cells, both in cultures derived from human embryonic stem cells and in the intact RPE monolayers from mouse eyes. We found that the pharmacological blockade of gap junctions eliminated electrical coupling between RPE cells, and that the blockade of gap junctions or Cx43 hemichannels significantly increased their input resistance. These results demonstrate that gap junctions function in the RPE not only as a means of molecular transport but also as a regulator of electrical excitability.


YSO-asiasanatbiofysiikkasolufysiologiaepiteelisolutverkkokalvo

Vapaat asiasanatbiophysics; cellular physiology


Liittyvät organisaatiot


Hankkeet, joissa julkaisu on tehty


OKM-raportointiKyllä

Raportointivuosi2022

JUFO-taso2


Viimeisin päivitys 2024-30-04 klo 17:36