A1 Journal article (refereed)
Branched-Chain Amino Acid Deprivation Decreases Lipid Oxidation and Lipogenesis in C2C12 Myotubes (2022)

Karvinen, S., Fachada, V., Sahinaho, U.-M., Pekkala, S., Lautaoja, J. H., Mäntyselkä, S., Permi, P., Hulmi, J. J., Silvennoinen, M., & Kainulainen, H. (2022). Branched-Chain Amino Acid Deprivation Decreases Lipid Oxidation and Lipogenesis in C2C12 Myotubes. Metabolites, 12(4), Article 328. https://doi.org/10.3390/metabo12040328

JYU authors or editors

Publication details

All authors or editorsKarvinen, Sira; Fachada, Vasco; Sahinaho, Ulla-Maria; Pekkala, Satu; Lautaoja, Juulia H.; Mäntyselkä, Sakari; Permi, Perttu; Hulmi, Juha J.; Silvennoinen, Mika; Kainulainen, Heikki

Journal or seriesMetabolites


Publication year2022

Publication date05/04/2022


Issue number4

Article number328

PublisherMDPI AG

Publication countrySwitzerland

Publication languageEnglish


Publication open accessOpenly available

Publication channel open accessOpen Access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/80585


Impaired lipid metabolism is a common risk factor underlying several metabolic diseases such as metabolic syndrome and type 2 diabetes. Branched-chain amino acids (BCAAs) that include valine, leucine and isoleucine have been proven to share a role in lipid metabolism and hence in maintaining metabolic health. We have previously introduced a hypothesis suggesting that BCAA degradation mechanistically connects to lipid oxidation and storage in skeletal muscle. To test our hypothesis, the present study examined the effects of BCAA deprivation and supplementation on lipid oxidation, lipogenesis and lipid droplet characteristics in murine C2C12 myotubes. In addition, the role of myotube contractions on cell metabolism was studied by utilizing in vitro skeletal-muscle-specific exercise-like electrical pulse stimulation (EPS). Our results showed that the deprivation of BCAAs decreased both lipid oxidation and lipogenesis in C2C12 myotubes. BCAA deprivation further diminished the number of lipid droplets in the EPS-treated myotubes. EPS decreased lipid oxidation especially when combined with high BCAA supplementation. Similar to BCAA deprivation, high BCAA supplementation also decreased lipid oxidation. The present results highlight the role of an adequate level of BCAAs in healthy lipid metabolism.

Keywordsmetabolic disorderslipid metabolismamino acidsadult-onset diabetesmuscle cells

Free keywordsmetabolic health; protein supplementation; skeletal muscle; electrical pulse stimulation; in vitro exercise; nuclear magnetic resonance spectroscopy

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Ministry reportingYes

Reporting Year2022

JUFO rating1

Last updated on 2024-15-06 at 22:26