A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Plasmid Viability Depends on the Ecological Setting of Hosts within a Multiplasmid Community (2022)
Given, C., Penttinen, R., & Jalasvuori, M. (2022). Plasmid Viability Depends on the Ecological Setting of Hosts within a Multiplasmid Community. Microbiology Spectrum, 10(2), e00133-22. https://doi.org/10.1128/spectrum.00133-22
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Given, Cindy; Penttinen, Reetta; Jalasvuori, Matti
Lehti tai sarja: Microbiology Spectrum
eISSN: 2165-0497
Julkaisuvuosi: 2022
Ilmestymispäivä: 13.04.2022
Volyymi: 10
Lehden numero: 2
Artikkelin sivunumerot: e00133-22
Kustantaja: American Society for Microbiology
Julkaisumaa: Yhdysvallat (USA)
Julkaisun kieli: englanti
DOI: https://doi.org/10.1128/spectrum.00133-22
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/80846
Rinnakkaistallenteen verkko-osoite (pre-print): https://doi.org/10.1101/2021.08.02.454727
Tiivistelmä
Plasmids are extrachromosomal genetic elements, some of which disperse horizontally between different strains and species of bacteria. They are a major factor in the dissemination of virulence factors and antibiotic resistance. Understanding the ecology of plasmids has a notable anthropocentric value, and therefore, the interactions between bacterial hosts and individual plasmids have been studied in detail. However, bacterial systems often carry multiple genetically distinct plasmids, but dynamics within these multiplasmid communities have remained unstudied. Here, we set to investigate the survival of 11 mobilizable or conjugative plasmids under five different conditions where the hosts had a differing ecological status in comparison to other bacteria in the system. The key incentive was to determine whether plasmid dynamics are reproducible and whether there are tradeoffs in plasmid fitness that stem from the ecological situation of their initial hosts. Growth rates and maximum population densities increased in all communities and treatments over the 42-day evolution experiment, although plasmid contents at the end varied notably. Large multiresistance-conferring plasmids were unfit when the community also contained smaller plasmids with fewer resistance genes. This suggests that restraining the use of a few antibiotics can make bacterial communities sensitive to others. In general, the presence or absence of antibiotic selection and plasmid-free hosts (of various fitnesses) has a notable influence on which plasmids survive. These tradeoffs in different settings can help explain, for example, why some resistance plasmids have an advantage during a rapid proliferation of antibiotic-sensitive pathogens whereas others dominate in alternative situations.
YSO-asiasanat: plasmidit; bakteerit; antibioottiresistenssi; virulenssi; taudinaiheuttajat; geenit; biotekniikka
Vapaat asiasanat: plasmid ecology; antibiotic resistance; multiresistance; plasmid evolution; plasmid stability; plasmid-mediated resistance
Liittyvät organisaatiot
OKM-raportointi: Kyllä
Raportointivuosi: 2022
JUFO-taso: 1
