A1 Journal article (refereed)
High-intensity interval training and moderate intensity training with exogenous adenosine counteract development of obesity in rats (2022)
L’entraînement par intervalles à haute intensité et l’entraînement à intensité modérée avec un apport d’adénosine exogène préviennent le développement de l’obésité chez le rat

Mirghani, S.J., Sharifian, S., Rafiei, M.M., Eslami, Z., Ghaderi, M., Moghanlou, A.E., Jaghoori, A., Mirghani, S.A., & Pekkala, S. (2022). High-intensity interval training and moderate intensity training with exogenous adenosine counteract development of obesity in rats. Science and Sports, 37(5-6), 477-485. https://doi.org/10.1016/j.scispo.2021.11.001

JYU authors or editors

Publication details

All authors or editors: Mirghani, S.J.; Sharifian, S.; Rafiei, M.M.; Eslami, Z.; Ghaderi, M.; Moghanlou, A.E.; Jaghoori, A.; Mirghani, S.A.; Pekkala, S.

Journal or series: Science and Sports

ISSN: 0765-1597

eISSN: 1778-4131

Publication year: 2022

Publication date: 28/02/2022

Volume: 37

Issue number: 5-6

Pages range: 477-485

Publisher: Elsevier BV

Publication country: France

Publication language: English

DOI: https://doi.org/10.1016/j.scispo.2021.11.001

Publication open access: Openly available

Publication channel open access: Partially open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/80708


High-Intensity Interval Training (HIIT) and Moderate Intensity Training (MIT) can combat the obesity epidemic. However, studies comparing their effects on obesity show controversial findings regarding weight loss. Adenosine has emerged as a possible, novel therapeutic agent to treat obesity, but more preclinical studies on its efficacy are needed. Therefore, the objectives of this study were to compare the effects of HIIT and MIT on obesity, and further to determine the dose-dependent effects of adenosine on body weight.

Equipment and methods
We induced obesity in male Wistar rats with high-fat diet (HFD) and monitored development of obesity by comparing the weight to rats on normal diet (ND). After 13 weeks of feeding, exercise training and intraperitoneal adenosine or placebo injections started. The animals were divided into the intervention groups based on equal average weight: (1) ND-1, 2) ND-2, (3) HFD, (4) HFD + placebo, (5) HFD + adenosine, (6) HFD + adenosine + HIIT, (7) HFD + placebo + HIIT, (8) HFD + adenosine + MIT, and (9) HFD + placebo + MIT. During the first six weeks of interventions, adenosine was injected at a dose of 0.2 mg/ml/kg and the following six weeks at 0.4 mg/ml/kg. Weight and feed consumption were determined, and energy intake was calculated as kJ/day.

The HFD started to cause obesity after 19 weeks of feeding. Both HIIT and MIT were equally effective in counteracting the HFD-induced weight gain, while no differences in the effects were found between the two exercise modes. Adenosine, independent of the dose, also prevented weight gain but did not enhance the effects of HIIT or MIT on weight. The obesity-counteracting effects of the treatments were not due to lower energy intake. In conclusion, our study shows that HIIT and MIT are equally effective to combat obesity, and further, provide preclinical evidence of that adenosine injections could be used to treat obesity.

Keywords: weight control; obesity; adenosine; exercise (people); interval training; animal disease models

Free keywords: exogenous adenosine; High-Intensity Interval Training (HIIT); Moderate Intensity Training (MIT); isocaloric; exercise; weight loss; energy intake; obesity; High Fat diet (HFD)

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Last updated on 2022-20-09 at 13:27