A1 Journal article (refereed)
Changes in cardiac proteome and metabolome following exposure to the PAHs retene and fluoranthene and their mixture in developing rainbow trout alevins (2022)

Eriksson, A. N., Rigaud, C., Rokka, A., Skaugen, M., Lihavainen, J. H., & Vehniäinen, E.-R. (2022). Changes in cardiac proteome and metabolome following exposure to the PAHs retene and fluoranthene and their mixture in developing rainbow trout alevins. Science of the Total Environment, 830, Article 154846. https://doi.org/10.1016/j.scitotenv.2022.154846

JYU authors or editors

Publication details

All authors or editors: Eriksson, Andreas N.M.; Rigaud, Cyril; Rokka, Anne; Skaugen, Morten; Lihavainen, Jenna H.; Vehniäinen, Eeva-Riikka

Journal or series: Science of the Total Environment

ISSN: 0048-9697

eISSN: 1879-1026

Publication year: 2022

Publication date: 26/03/2022

Volume: 830

Article number: 154846

Publisher: Elsevier BV

Publication country: Netherlands

Publication language: English

DOI: https://doi.org/10.1016/j.scitotenv.2022.154846

Publication open access: Openly available

Publication channel open access: Partially open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/81270

Abstract

Exposure to polycyclic aromatic hydrocarbons (PAHs) is known to affect developing organisms. Utilization of different omics-based technologies and approaches could therefore provide a base for the discovery of novel mechanisms of PAH induced development of toxicity. To this aim, we investigated how exposure towards two PAHs with different toxicity mechanisms: retene (an aryl hydrocarbon receptor 2 (Ahr2) agonist), and fluoranthene (a weak Ahr2 agonist and cytochrome P450 inhibitor (Cyp1a)), either alone or as a mixture, affected the cardiac proteome and metabolome in newly hatched rainbow trout alevins (Oncorhynchus mykiss). In total, we identified 65 and 82 differently expressed proteins (DEPs) across all treatments compared to control (DMSO) after 7 and 14 days of exposure. Exposure to fluoranthene altered the expression of 11 and 19 proteins, retene 29 and 23, while the mixture affected 44 and 82 DEPs by Days 7 and 14, respectively. In contrast, only 5 significantly affected metabolites were identified. Pathway over-representation analysis identified exposure-specific activation of phase II metabolic processes, which were accompanied with exposure-specific body burden profiles. The proteomic data highlights that exposure to the mixture increased oxidative stress, altered iron metabolism and impaired coagulation capacity. Additionally, depletion of several mini-chromosome maintenance components, in combination with depletion of several intermediate filaments and microtubules, among alevins exposed to the mixture, suggests compromised cellular integrity and reduced rate of mitosis, whereby affecting heart growth and development. Furthermore, the combination of proteomic and metabolomic data indicates altered energy metabolism, as per amino acid catabolism among mixture exposed alevins; plausibly compensatory mechanisms as to counteract reduced absorption and consumption of yolk. When considered as a whole, proteomic and metabolomic data, in relation to apical effects on the whole organism, provides additional insight into PAH toxicity and the effects of exposure on heart structure and molecular processes.

Keywords: ecotoxicology; developmental biology; heart; proteomics; metabolism; PAH compounds; rainbow trout

Free keywords: PROTEOMICS; metabolomics; PAH; mixture; developmental toxicology; rainbow trout

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Ministry reporting: Yes

VIRTA submission year: 2022

JUFO rating: 2