A1 Journal article (refereed)
Enzyme-assisted extraction of anthocyanins and other phenolic compounds from blackcurrant (Ribes nigrum L.) press cake : From processing to bioactivities (2022)
Granato, D., Fidelis, M., Haapakoski, M., dos Santos Lima, A., Viil, J., Hellström, J., Rätsep, R., Kaldmäe, H., Bleive, U., Azevedo, L., Marjomäki, V., Zharkovsky, A., & Pap, N. (2022). Enzyme-assisted extraction of anthocyanins and other phenolic compounds from blackcurrant (Ribes nigrum L.) press cake : From processing to bioactivities. Food Chemistry, 391, Article 133240. https://doi.org/10.1016/j.foodchem.2022.133240
JYU authors or editors
Publication details
All authors or editors: Granato, Daniel; Fidelis, Marina; Haapakoski, Marjo; dos Santos Lima, Amanda; Viil, Janeli; Hellström, Jarkko; Rätsep, Reelika; Kaldmäe, Hedi; Bleive, Uko; Azevedo, Luciana; et al.
Journal or series: Food Chemistry
ISSN: 0308-8146
eISSN: 1873-7072
Publication year: 2022
Publication date: 18/05/2022
Volume: 391
Article number: 133240
Publisher: Elsevier Ltd.
Publication country: United Kingdom
Publication language: English
DOI: https://doi.org/10.1016/j.foodchem.2022.133240
Publication open access: Openly available
Publication channel open access: Partially open access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/82317
Abstract
The effects of commercial enzymes (pectinases, cellulases, beta-1-3-glucanases, and pectin lyases) on the recovery of anthocyanins and polyphenols from blackcurrant press cake were studied considering two solid:solvent ratios (1:10 and 1:4 w/v). β-glucanase enabled the recovery of the highest total phenolic content – 1142 mg/100 g, and the extraction of anthocyanins was similar using all enzymes (∼400 mg/100 g). The use of cellulases and pectinases enhanced the extraction of antioxidants (DPPH − 1080 mg/100 g; CUPRAC – 3697 mg/100 g). The freeze-dried extracts presented antioxidant potential (CUPRAC, DPPH), which was associated with their biological effects in different systems: antiviral activity against both non-enveloped viruses (enterovirus coxsackievirus A-9) and enveloped coronaviruses (HCoV-OC43), and cytotoxicity towards cancer cells (A549 and HCT8). No cytotoxic effects on normal human lung fibroblast (IMR90) were observed, and no anti-inflammatory activity was detected in lipopolysaccharides-treated murine immortalised microglial cells.
Keywords: naturally occurring substances; bioactive compounds; antioxidants; antimicrobial compounds; phenols; polyphenols; anthocyanins; recovery (recapture); extraction (chemistry); black currant; by-products; circular economy
Free keywords: blackcurrant side-streams; antioxidant activity; antiviral activity; anti-inflammatory activity; antiproliferative activity; circular economy; polyphenols
Contributing organizations
Ministry reporting: Yes
Reporting Year: 2022
JUFO rating: 2
