A1 Journal article (refereed)
Enzyme-assisted extraction of anthocyanins and other phenolic compounds from blackcurrant (Ribes nigrum L.) press cake : From processing to bioactivities (2022)

Granato, D., Fidelis, M., Haapakoski, M., dos Santos Lima, A., Viil, J., Hellström, J., Rätsep, R., Kaldmäe, H., Bleive, U., Azevedo, L., Marjomäki, V., Zharkovsky, A., & Pap, N. (2022). Enzyme-assisted extraction of anthocyanins and other phenolic compounds from blackcurrant (Ribes nigrum L.) press cake : From processing to bioactivities. Food Chemistry, 391, Article 133240. https://doi.org/10.1016/j.foodchem.2022.133240

JYU authors or editors

Publication details

All authors or editors: Granato, Daniel; Fidelis, Marina; Haapakoski, Marjo; dos Santos Lima, Amanda; Viil, Janeli; Hellström, Jarkko; Rätsep, Reelika; Kaldmäe, Hedi; Bleive, Uko; Azevedo, Luciana; et al.

Journal or series: Food Chemistry

ISSN: 0308-8146

eISSN: 1873-7072

Publication year: 2022

Publication date: 18/05/2022

Volume: 391

Article number: 133240

Publisher: Elsevier Ltd.

Publication country: United Kingdom

Publication language: English

DOI: https://doi.org/10.1016/j.foodchem.2022.133240

Publication open access: Openly available

Publication channel open access: Partially open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/82317

Abstract

The effects of commercial enzymes (pectinases, cellulases, beta-1-3-glucanases, and pectin lyases) on the recovery of anthocyanins and polyphenols from blackcurrant press cake were studied considering two solid:solvent ratios (1:10 and 1:4 w/v). β-glucanase enabled the recovery of the highest total phenolic content – 1142 mg/100 g, and the extraction of anthocyanins was similar using all enzymes (∼400 mg/100 g). The use of cellulases and pectinases enhanced the extraction of antioxidants (DPPH − 1080 mg/100 g; CUPRAC – 3697 mg/100 g). The freeze-dried extracts presented antioxidant potential (CUPRAC, DPPH), which was associated with their biological effects in different systems: antiviral activity against both non-enveloped viruses (enterovirus coxsackievirus A-9) and enveloped coronaviruses (HCoV-OC43), and cytotoxicity towards cancer cells (A549 and HCT8). No cytotoxic effects on normal human lung fibroblast (IMR90) were observed, and no anti-inflammatory activity was detected in lipopolysaccharides-treated murine immortalised microglial cells.

Keywords: naturally occurring substances; bioactive compounds; antioxidants; antimicrobial compounds; phenols; polyphenols; anthocyanins; recovery (recapture); extraction (chemistry); black currant; by-products; circular economy

Free keywords: blackcurrant side-streams; antioxidant activity; antiviral activity; anti-inflammatory activity; antiproliferative activity; circular economy; polyphenols

Contributing organizations

Ministry reporting: Yes

Reporting Year: 2022

Preliminary JUFO rating: 2