A1 Journal article (refereed)
Conserved histidine and tyrosine determine spectral responses through the water network in Deinococcus radiodurans phytochrome (2022)

Lehtivuori, H., Rumfeldt, J., Mustalahti, S., Kurkinen, S., & Takala, H. (2022). Conserved histidine and tyrosine determine spectral responses through the water network in Deinococcus radiodurans phytochrome. Photochemical and Photobiological Sciences, 21(11), 1975-1989. https://doi.org/10.1007/s43630-022-00272-6

JYU authors or editors

Publication details

All authors or editors: Lehtivuori, Heli; Rumfeldt, Jessica; Mustalahti, Satu; Kurkinen, Sami; Takala, Heikki

Journal or series: Photochemical and Photobiological Sciences

ISSN: 1474-905X

eISSN: 1474-9092

Publication year: 2022

Publication date: 29/07/2022

Volume: 21

Issue number: 11

Pages range: 1975-1989

Publisher: Springer Science and Business Media LLC

Publication country: Germany

Publication language: English

DOI: https://doi.org/10.1007/s43630-022-00272-6

Publication open access: Openly available

Publication channel open access: Partially open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/82490

Abstract

Phytochromes are red light-sensing photoreceptor proteins that bind a bilin chromophore. Here, we investigate the role of a conserved histidine (H260) and tyrosine (Y263) in the chromophore-binding domain (CBD) of Deinococcus radiodurans phytochrome (DrBphP). Using crystallography, we show that in the H260A variant, the missing imidazole side chain leads to increased water content in the binding pocket. On the other hand, Y263F mutation reduces the water occupancy around the chromophore. Together, these changes in water coordination alter the protonation and spectroscopic properties of the biliverdin. These results pinpoint the importance of this conserved histidine and tyrosine, and the related water network, for the function and applications of phytochromes.

Keywords: bacteria; proteins; radiation; light (electromagnetic radiation); photochemistry; photobiology; spectroscopy; crystallography

Free keywords: spectral responses; water network; biliverdin protonation; phytochrome structure

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Ministry reporting: Yes

Reporting Year: 2022

Preliminary JUFO rating: 1