A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
The structural effect between the output module and chromophore-binding domain is a two-way street via the hairpin extension (2022)
Kurttila, M., Etzl, S., Rumfeldt, J., Takala, H., Galler, N., Winkler, A., & Ihalainen, J. A. (2022). The structural effect between the output module and chromophore-binding domain is a two-way street via the hairpin extension. Photochemical and Photobiological Sciences, 21(11), 1881-1894. https://doi.org/10.1007/s43630-022-00265-5
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Kurttila, Moona; Etzl, Stefan; Rumfeldt, Jessica; Takala, Heikki; Galler, Nadine; Winkler, Andreas; Ihalainen, Janne A.
Lehti tai sarja: Photochemical and Photobiological Sciences
ISSN: 1474-905X
eISSN: 1474-9092
Julkaisuvuosi: 2022
Ilmestymispäivä: 19.08.2022
Volyymi: 21
Lehden numero: 11
Artikkelin sivunumerot: 1881-1894
Kustantaja: Springer VS
Julkaisumaa: Saksa
Julkaisun kieli: englanti
DOI: https://doi.org/10.1007/s43630-022-00265-5
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Osittain avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/82776
Lisätietoja: This publication is dedicated to Prof. Silvia E. Braslavsky, a pioneer in photobiology and photobiophysics, on the occasion of her 80th birthday.
Tiivistelmä
Signal transduction typically starts with either ligand binding or cofactor activation, eventually affecting biological activities in the cell. In red light-sensing phytochromes, isomerization of the bilin chromophore results in regulation of the activity of diverse output modules. During this process, several structural elements and chemical events influence signal propagation. In our study, we have studied the full-length bacteriophytochrome from Deinococcus radiodurans as well as a previously generated optogenetic tool where the native histidine kinase output module has been replaced with an adenylate cyclase. We show that the composition of the output module influences the stability of the hairpin extension. The hairpin, often referred as the PHY tongue, is one of the central structural elements for signal transduction. It extends from a distinct domain establishing close contacts with the chromophore binding site. If the coupling between these interactions is disrupted, the dynamic range of the enzymatic regulation is reduced. Our study highlights the complex conformational properties of the hairpin extension as a bidirectional link between the chromophore-binding site and the output module, as well as functional properties of diverse output modules.
YSO-asiasanat: solut; biologinen aktiivisuus; proteiinit; signaalit; valo; fotobiologia; biotieteet
Vapaat asiasanat: punavalo
Liittyvät organisaatiot
Hankkeet, joissa julkaisu on tehty
- Geenit vai ympäristö: Kuinka proteiinien ympäristö vaikuttaa niiden toimintaan
- Ihalainen, Janne
- Suomen Akatemia
- Fytokromipohjaiset moduulit – toiminta ja sovellukset
- Takala, Heikki
- Suomen Akatemia
OKM-raportointi: Kyllä
Raportointivuosi: 2022
JUFO-taso: 1