A1 Journal article (refereed)
The structural effect between the output module and chromophore-binding domain is a two-way street via the hairpin extension (2022)

Kurttila, M., Etzl, S., Rumfeldt, J., Takala, H., Galler, N., Winkler, A., & Ihalainen, J. A. (2022). The structural effect between the output module and chromophore-binding domain is a two-way street via the hairpin extension. Photochemical and Photobiological Sciences, 21(11), 1881-1894. https://doi.org/10.1007/s43630-022-00265-5

JYU authors or editors

Kurttila, Moona
Rumfeldt, Jessica
Takala, Heikki
Ihalainen, Janne

Publication details

All authors or editors: Kurttila, Moona; Etzl, Stefan; Rumfeldt, Jessica; Takala, Heikki; Galler, Nadine; Winkler, Andreas; Ihalainen, Janne A.

Journal or series: Photochemical and Photobiological Sciences

ISSN: 1474-905X

eISSN: 1474-9092

Publication year: 2022

Publication date: 19/08/2022

Volume: 21

Issue number: 11

Pages range: 1881-1894

Publisher: Springer VS

Publication country: Germany

Publication language: English

DOI: https://doi.org/10.1007/s43630-022-00265-5

Publication open access: Openly available

Publication channel open access: Partially open access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/82776

Additional information: This publication is dedicated to Prof. Silvia E. Braslavsky, a pioneer in photobiology and photobiophysics, on the occasion of her 80th birthday.

Abstract

Signal transduction typically starts with either ligand binding or cofactor activation, eventually affecting biological activities in the cell. In red light-sensing phytochromes, isomerization of the bilin chromophore results in regulation of the activity of diverse output modules. During this process, several structural elements and chemical events influence signal propagation. In our study, we have studied the full-length bacteriophytochrome from Deinococcus radiodurans as well as a previously generated optogenetic tool where the native histidine kinase output module has been replaced with an adenylate cyclase. We show that the composition of the output module influences the stability of the hairpin extension. The hairpin, often referred as the PHY tongue, is one of the central structural elements for signal transduction. It extends from a distinct domain establishing close contacts with the chromophore binding site. If the coupling between these interactions is disrupted, the dynamic range of the enzymatic regulation is reduced. Our study highlights the complex conformational properties of the hairpin extension as a bidirectional link between the chromophore-binding site and the output module, as well as functional properties of diverse output modules.

Keywords: cells; biological activity; proteins; signals; light (electromagnetic radiation); photobiology; biosciences