A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells (2022)


Chen, J.-H., Vanslembrouck, B., Ekman, A., Aho, V., Larabell, C. A., Le Gros, M. A., Vihinen-Ranta, M., & Weinhardt, V. (2022). Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells. Viruses, 14(12), 2651. https://doi.org/10.3390/v14122651


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajatChen, Jian-Hua; Vanslembrouck, Bieke; Ekman, Axel; Aho, Vesa; Larabell, Carolyn A.; Le Gros, Mark A.; Vihinen-Ranta, Maija; Weinhardt, Venera

Lehti tai sarjaViruses

eISSN1999-4915

Julkaisuvuosi2022

Ilmestymispäivä27.11.2022

Volyymi14

Lehden numero12

Artikkelin sivunumerot2651

KustantajaMDPI AG

JulkaisumaaSveitsi

Julkaisun kielienglanti

DOIhttps://doi.org/10.3390/v14122651

Julkaisun avoin saatavuusAvoimesti saatavilla

Julkaisukanavan avoin saatavuusKokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX)https://jyx.jyu.fi/handle/123456789/84688


Tiivistelmä

Upon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. Scouting gross cellular changes with fluorescent microscopy is only possible for well-established viruses, where fluorescent tagging is developed. Soft X-ray tomography (SXT) offers 3D imaging of entire cells without the need for chemical fixation or labeling. Here, we use full-rotation SXT to visualize entire human B cells infected by the herpes simplex virus 1 (HSV-1). We have mapped the temporospatial remodeling of cells during the infection and observed changes in cellular structures, such as the presence of cytoplasmic stress granules and multivesicular structures, formation of nuclear virus-induced dense bodies, and aggregates of capsids. Our results demonstrate the power of SXT imaging for scouting virus-induced changes in infected cells and understanding the orchestration of virus-host remodeling quantitatively.


YSO-asiasanatviruksetsolutisäntäsoluttomografiaröntgenkuvausmikroskopiainfektiotsolubiologiaherpesvirusinfektiotherpesviruksetherpes simplex -virus

Vapaat asiasanatX-ray tomography; soft X-rays; infection imaging; HSV-1; cell mapping; cryo imaging


Liittyvät organisaatiot


Hankkeet, joissa julkaisu on tehty


OKM-raportointiKyllä

Raportointivuosi2022

JUFO-taso1


Viimeisin päivitys 2024-15-06 klo 22:06