A1 Journal article (refereed)
Glyphosate-based herbicide use affects individual microbial taxa in strawberry endosphere but not the microbial community composition (2023)


Mathew, S. A., Fuchs, B., Nissinen, R., Helander, M., Puigbò, P., Saikkonen, K., & Muola, A. (2023). Glyphosate-based herbicide use affects individual microbial taxa in strawberry endosphere but not the microbial community composition. Journal of Applied Microbiology, 134(2), Article lxad006. https://doi.org/10.1093/jambio/lxad006


JYU authors or editors


Publication details

All authors or editorsMathew, Suni Anie; Fuchs, Benjamin; Nissinen, Riitta; Helander, Marjo; Puigbò, Pere; Saikkonen, Kari; Muola, Anne

Journal or seriesJournal of Applied Microbiology

ISSN1364-5072

eISSN1365-2672

Publication year2023

Publication date13/01/2023

Volume134

Issue number2

Article numberlxad006

PublisherOxford University Press (OUP)

Publication countryUnited Kingdom

Publication languageEnglish

DOIhttps://doi.org/10.1093/jambio/lxad006

Publication open accessOpenly available

Publication channel open accessPartially open access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/85572


Abstract

Aims
In a field study, the effects of treatments of glyphosate-based herbicides (GBHs) in soil, alone and in combination with phosphate fertilizer, were examined on the performance and endophytic microbiota of garden strawberry.

Methods and Results
The root and leaf endophytic microbiota of garden strawberries grown in GBH-treated and untreated soil, with and without phosphate fertilizer were analyzed. Next, bioinformatics analysis on the type of 5-enolpyruvylshikimate-3-phosphate synthase enzyme was conducted to assess the potential sensitivity of strawberry-associated bacteria and fungi to glyphosate, and to compare the results with field observations. GBH treatments altered the abundance and/or frequency of several operational taxonomic units (OTUs), especially those of root-associated fungi and bacteria. These changes were partly related to their sensitivity to glyphosate. Still, GBH treatments did not shape the overall community structure of strawberry microbiota or affect plant performance. Phosphate fertilizer increased the abundance of both glyphosate-resistant and glyphosate-sensitive bacterial OTUs, regardless of the GBH treatments.

Conclusions
These findings demonstrate that although the overall community structure of strawberry endophytic microbes is not affected by GBH use, some individual taxa are.


Keywordsendophytesherbicidesmicrobiomephosphatic fertilizers

Free keywordsEPSPS; Fragaria X ananassa; herbicide; roundup; endophyte; microbiota; phosphate fertilizer; plant performance


Contributing organizations


Ministry reportingYes

Reporting Year2023

JUFO rating1


Last updated on 2024-02-07 at 23:27