A2 Review article, Literature review, Systematic review
Mimicking exercise in vitro - effects of myotube contractions and mechanical stretch on omics (2023)

Lautaoja, J. H., Turner, D. C., Sharples, A. P., Kivelä, R., Pekkala, S., Hulmi, J. J., & Ylä-Outinen, L. (2023). Mimicking exercise in vitro - effects of myotube contractions and mechanical stretch on omics. American Journal of Physiology : Cell Physiology, 324(4), C886-C892. https://doi.org/10.1152/ajpcell.00586.2022

JYU authors or editors

Publication details

All authors or editors: Lautaoja, Juulia H.; Turner, Daniel C.; Sharples, Adam P.; Kivelä, Riikka; Pekkala, Satu; Hulmi, Juha J.; Ylä-Outinen, Laura

Journal or series: American Journal of Physiology : Cell Physiology

ISSN: 0363-6143

eISSN: 1522-1563

Publication year: 2023

Publication date: 07/03/2023

Volume: 324

Issue number: 4

Pages range: C886-C892

Publisher: American Physiological Society

Publication country: United States

Publication language: English

DOI: https://doi.org/10.1152/ajpcell.00586.2022

Publication open access: Not open

Publication channel open access: 

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/86478

Abstract

The number of studies using skeletal muscle (SkM) cell culture models to study exercise in vitro are rapidly expanding. Progressively, more comprehensive analysis methods, such as different omics approaches including transcriptomics, proteomics and metabolomics have been used to examine the intra- and extracellular molecular responses to exercise mimicking stimuli in cultured myotubes. Among other techniques, exercise-like electrical pulse stimulation (EL-EPS) and mechanical stretch of SkM cells are the two most commonly used methods to mimic exercise in vitro. In this mini-review we focus on these two approaches and their effects on the omics of myotubes and/or cell culture media. Furthermore, besides traditional two-dimensional (2D) methods, the use of three-dimensional (3D) SkM approaches are increasing in the field of in vitro exercise mimicry. Our aim with this mini-review is to provide the reader with an up-to-date overview of the 2D and 3D models and the use of omics approaches to study the molecular response to exercise in vitro.

Keywords: muscle cells; cell physiology; research methods; in vitro method; cell culture; transcriptome; proteomics; metabolic products

Free keywords: electrical pulse stimulation; skeletal muscle; transcriptomics; proteomics; metabolomics

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Ministry reporting: Yes

VIRTA submission year: 2023

JUFO rating: 2