A1 Journal article (refereed)
Creation of ordered 3D tubes out of DNA origami lattices (2023)


Parikka, J. M., Järvinen, H., Sokołowska, K., Ruokolainen, V., Markešević, N., Natarajan, A. K., Vihinen-Ranta, M., Kuzyk, A., Tapio, K., & Toppari, J. J. (2023). Creation of ordered 3D tubes out of DNA origami lattices. Nanoscale, 15(17), 7772-7780. https://doi.org/10.1039/D2NR06001A


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Publication details

All authors or editorsParikka, Johannes M.; Järvinen, Heini; Sokołowska, Karolina; Ruokolainen, Visa; Markešević, Nemanja; Natarajan, Ashwin K.; Vihinen-Ranta, Maija; Kuzyk, Anton; Tapio, Kosti; Toppari, J. Jussi

Journal or seriesNanoscale

ISSN2040-3364

eISSN2040-3372

Publication year2023

Publication date28/03/2023

Volume15

Issue number17

Pages range7772-7780

PublisherRoyal Society of Chemistry

Publication countryUnited Kingdom

Publication languageEnglish

DOIhttps://doi.org/10.1039/D2NR06001A

Publication open accessOpenly available

Publication channel open accessPartially open access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/86593


Abstract

Hierarchical self-assembly of nanostructures with addressable complexity has been a promising route for realizing novel functional materials. Traditionally, the fabrication of such structures on a large scale has been achievable using top-down methods but with the cost of complexity of the fabrication equipment versus resolution and limitation mainly to 2D structures. More recently bottom-up methods using molecules like DNA have gained attention due to the advantages of low fabrication costs, high resolution and simplicity in an extension of the methods to the third dimension. One of the more promising bottom-up techniques is DNA origami due to the robust self-assembly of arbitrarily shaped nanostructures with feature sizes down to a few nanometers. Here, we show that under specific ionic conditions of the buffer, the employed plus-shaped, blunt-ended Seeman tile (ST) origami forms elongated, ordered 2D lattices, which are further rolled into 3D tubes in solution. Imaging structures on a surface by atomic force microscopy reveals ribbon-like structures, with single or double layers of the origami lattice. Further studies of the double-layered structures in a liquid state by confocal microscopy and cryo-TEM revealed elongated tube structures with a relatively uniform width but with a varying length. Through meticulous study, we concluded that the assembly process of these 3D DNA origami tubes is heavily dependent on the concentration of both mono- and divalent cations. In particular, nickel seems to act as a trigger for the formation of the tubular assemblies in liquid.


KeywordsnanostructuresDNAfunctional materials


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Ministry reportingYes

Reporting Year2023

JUFO rating3


Last updated on 2024-02-07 at 23:46