B2 Book section
Protein Coating of DNA Origami (2023)


Ijäs, H., Kostiainen, M. A., & Linko, V. (2023). Protein Coating of DNA Origami. In J. Valero (Ed.), DNA and RNA Origami : Methods and Protocols (pp. 195-207). Humana Press. Methods in Molecular Biology, 2639. https://doi.org/10.1007/978-1-0716-3028-0_12


JYU authors or editors


Publication details

All authors or editorsIjäs, Heini; Kostiainen, Mauri A.; Linko, Veikko

Parent publicationDNA and RNA Origami : Methods and Protocols

Parent publication editorsValero, Julián

ISBN978-1-0716-3027-3

eISBN978-1-0716-3028-0

Journal or seriesMethods in Molecular Biology

ISSN1064-3745

eISSN1940-6029

Publication year2023

Publication date12/05/2023

Number in series2639

Pages range195-207

Number of pages in the book353

PublisherHumana Press

Place of PublicationNew York

Publication countryUnited States

Publication languageEnglish

DOIhttps://doi.org/10.1007/978-1-0716-3028-0_12

Publication open accessNot open

Publication channel open access


Abstract

DNA origami has emerged as a common technique to create custom two- (2D) and three-dimensional (3D) structures at the nanoscale. These DNA nanostructures have already proven useful in development of many biotechnological tools; however, there are still challenges that cast a shadow over the otherwise bright future of biomedical uses of these DNA objects. The rather obvious obstacles in harnessing DNA origami as drug-delivery vehicles and/or smart biodevices are related to their debatable stability in biologically relevant media, especially in physiological low-cation and endonuclease-rich conditions, relatively poor transfection rates, and, although biocompatible by nature, their unpredictable compatibility with the immune system. Here we demonstrate a technique for coating DNA origami with albumin proteins for enhancing their pharmacokinetic properties. To facilitate protective coating, a synthesized positively charged dendron was linked to bovine serum albumin (BSA) through a covalent maleimide-cysteine bonding, and then the purified dendron-protein conjugates were let to assemble on the negatively charged surface of DNA origami via electrostatic interaction. The resulted BSA-dendron conjugate-coated DNA origami showed improved transfection, high resistance against endonuclease digestion, and significantly enhanced immunocompatibility compared to bare DNA origami. Furthermore, our proposed coating strategy can be considered highly versatile as a maleimide-modified dendron serving as a synthetic DNA-binding domain can be linked to any protein with an available cysteine site.


KeywordsDNAnucleic acidsnanotechnologynanostructuresself-assembly (chemistry)proteins

Free keywordsnucleic acids; DNA nanotechnology; DNA nanostructures; self-assembly; electrostatic interaction; protein; dendron; drug delivery; DNA origami stability


Contributing organizations


Ministry reportingYes

Reporting Year2023


Last updated on 2024-15-05 at 13:05