B2 Book section
Protein Coating of DNA Origami (2023)

Ijäs, H., Kostiainen, M. A., & Linko, V. (2023). Protein Coating of DNA Origami. In J. Valero (Ed.), DNA and RNA Origami : Methods and Protocols (pp. 195-207). Humana Press. Methods in Molecular Biology, 2639. https://doi.org/10.1007/978-1-0716-3028-0_12

JYU authors or editors

Publication details

All authors or editors: Ijäs, Heini; Kostiainen, Mauri A.; Linko, Veikko

Parent publication: DNA and RNA Origami : Methods and Protocols

Parent publication editors: Valero, Julián

ISBN: 978-1-0716-3027-3

eISBN: 978-1-0716-3028-0

Journal or series: Methods in Molecular Biology

ISSN: 1064-3745

eISSN: 1940-6029

Publication year: 2023

Publication date: 12/05/2023

Number in series: 2639

Pages range: 195-207

Number of pages in the book: 353

Publisher: Humana Press

Place of Publication: New York

Publication country: United States

Publication language: English

DOI: https://doi.org/10.1007/978-1-0716-3028-0_12

Publication open access: Not open

Publication channel open access: 

Abstract

DNA origami has emerged as a common technique to create custom two- (2D) and three-dimensional (3D) structures at the nanoscale. These DNA nanostructures have already proven useful in development of many biotechnological tools; however, there are still challenges that cast a shadow over the otherwise bright future of biomedical uses of these DNA objects. The rather obvious obstacles in harnessing DNA origami as drug-delivery vehicles and/or smart biodevices are related to their debatable stability in biologically relevant media, especially in physiological low-cation and endonuclease-rich conditions, relatively poor transfection rates, and, although biocompatible by nature, their unpredictable compatibility with the immune system. Here we demonstrate a technique for coating DNA origami with albumin proteins for enhancing their pharmacokinetic properties. To facilitate protective coating, a synthesized positively charged dendron was linked to bovine serum albumin (BSA) through a covalent maleimide-cysteine bonding, and then the purified dendron-protein conjugates were let to assemble on the negatively charged surface of DNA origami via electrostatic interaction. The resulted BSA-dendron conjugate-coated DNA origami showed improved transfection, high resistance against endonuclease digestion, and significantly enhanced immunocompatibility compared to bare DNA origami. Furthermore, our proposed coating strategy can be considered highly versatile as a maleimide-modified dendron serving as a synthetic DNA-binding domain can be linked to any protein with an available cysteine site.

Keywords: DNA; nucleic acids; nanotechnology; nanostructures; self-assembly (chemistry); proteins

Free keywords: nucleic acids; DNA nanotechnology; DNA nanostructures; self-assembly; electrostatic interaction; protein; dendron; drug delivery; DNA origami stability

Contributing organizations

Ministry reporting: Yes

VIRTA submission year: 2023