A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Protocols for sample preparation and compound-specific stable-isotope analyses (δ2H, δ13C) of fatty acids in biological and environmental samples (2023)


Pilecky, M., Wassenaar, L. I., Taipale, S., & Kainz, M. J. (2023). Protocols for sample preparation and compound-specific stable-isotope analyses (δ2H, δ13C) of fatty acids in biological and environmental samples. MethodsX, 11, Article 102283. https://doi.org/10.1016/j.mex.2023.102283


JYU-tekijät tai -toimittajat


Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajatPilecky, Matthias; Wassenaar, Leonard I.; Taipale, Sami; Kainz, Martin J.

Lehti tai sarjaMethodsX

eISSN2215-0161

Julkaisuvuosi2023

Ilmestymispäivä11.07.2023

Volyymi11

Artikkelinumero102283

KustantajaElsevier BV

JulkaisumaaAlankomaat

Julkaisun kielienglanti

DOIhttps://doi.org/10.1016/j.mex.2023.102283

Julkaisun avoin saatavuusAvoimesti saatavilla

Julkaisukanavan avoin saatavuusKokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX)https://jyx.jyu.fi/handle/123456789/88772


Tiivistelmä

Compound-specific stable-isotope analysis (CSIA) of fatty acids is a powerful tool to better understand the trophic transfer of fatty acids and their biochemical fate in and across ecosystems, including tracing animal migration and understanding physiological processes. The non-exchangeable nature of C–H bonds in acyl chains, hydrogen (𝛿2H) and carbon (𝛿13C) stable-isotope values of fatty acids (FA) provide independent information about the origins of fatty acids. Several technical obstacles must be overcome to ensure accurate and reproducible measurements of FA-CSIA can be made. This protocol describes the sample preparation process for successful stable-isotope analyses of fatty acids obtained from environmental and biological samples. Numerous techniques for the preanalytical processing of fatty acid samples are available, and these often have minimal impact on 𝛿 values. Here, we provide an in-depth guide detailing our well-established laboratory protocols, ranging from the initial sample preparation, lipid extraction, and transmethylation to the instrumental arrangement, data collection, and analysis.

• Protocol from obtaining a sample to standardized fatty acid specific 𝛿2H and 𝛿13C values.
• Separate GC analysis procedures for C and H are recommended for optimal performance.


YSO-asiasanatrasvahapothiilivetyisotoopitisotooppianalyysilaboratoriotekniikkanäytteet

Vapaat asiasanatcarbon isotopes; deuterium; fatty acids; GC-IRMS; stable-isotopes; CSIA


Liittyvät organisaatiot


OKM-raportointiKyllä

Raportointivuosi2023

Alustava JUFO-taso0


Viimeisin päivitys 2024-18-02 klo 18:25