A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
From maternal glucocorticoid and thyroid hormones to epigenetic regulation of offspring gene expression : An experimental study in a wild bird species (2023)
Hukkanen, M., Hsu, B., Cossin‐Sevrin, N., Crombecque, M., Delaunay, A., Hollmen, L., Kaukonen, R., Konki, M., Lund, R., Marciau, C., Stier, A., & Ruuskanen, S. (2023). From maternal glucocorticoid and thyroid hormones to epigenetic regulation of offspring gene expression : An experimental study in a wild bird species. Evolutionary Applications, 16(10), 1753-1769. https://doi.org/10.1111/eva.13598
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Hukkanen, Mikaela; Hsu, Bin‐Yan; Cossin‐Sevrin, Nina; Crombecque, Mélanie; Delaunay, Axelle; Hollmen, Lotta; Kaukonen, Riina; Konki, Mikko; Lund, Riikka; Marciau, Coline; et al.
Lehti tai sarja: Evolutionary Applications
eISSN: 1752-4571
Julkaisuvuosi: 2023
Ilmestymispäivä: 03.10.2023
Volyymi: 16
Lehden numero: 10
Artikkelin sivunumerot: 1753-1769
Kustantaja: Wiley
Julkaisumaa: Britannia
Julkaisun kieli: englanti
DOI: https://doi.org/10.1111/eva.13598
Linkki tutkimusaineistoon: https://doi.org/10.6084/m9.figshare.22153001
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/89511
Rinnakkaistallenteen verkko-osoite (pre-print): https://www.biorxiv.org/content/10.1101/2023.03.07.531470v1
Tiivistelmä
Offspring phenotype at birth is determined by its genotype and the prenatal environment including exposure to maternal hormones. Variation in both maternal glucocorticoids and thyroid hormones can affect offspring phenotype, but the underlying molecular mechanisms, especially those contributing to long-lasting effects, remain unclear. Epigenetic changes (such as DNA methylation) have been postulated as mediators of long-lasting effects of early-life environment. In this study, we determined the effects of elevated prenatal glucocorticoid and thyroid hormones on handling stress response (breath rate) as well as DNA methylation and gene expression of glucocorticoid receptor (GR) and thyroid hormone receptor (THR) in great tits (Parus major). Eggs were injected before incubation onset with corticosterone (the main avian glucocorticoid) and/or thyroid hormones (thyroxine and triiodothyronine) to simulate variation in maternal hormone deposition. Breath rate during handling and gene expression of GR and THR were evaluated 14 days after hatching. Methylation status of GR and THR genes was analyzed from the longitudinal blood cells sampled 7 and 14 days after hatching, as well as the following autumn. Elevated prenatal corticosterone level significantly increased the breath rate during handling, indicating an enhanced metabolic stress response. Prenatal corticosterone manipulation had CpG-site-specific effects on DNA methylation at the GR putative promoter region, while it did not significantly affect GR gene expression. GR expression was negatively associated with earlier hatching date and chick size. THR methylation or expression did not exhibit any significant relationship with the hormonal treatments or the examined covariates, suggesting that TH signaling may be more robust due to its crucial role in development. This study provides some support to the hypothesis suggesting that maternal corticosterone may influence offspring metabolic stress response via epigenetic alterations, yet their possible adaptive role in optimizing offspring phenotype to the prevailing conditions, context-dependency, and the underlying molecular interplay needs further research.
YSO-asiasanat: epigenetiikka; geenit; geeniekspressio; fenotyyppi; metylaatio; DNA-metylaatio; hormonit; glukokortikoidit; linnut; talitiainen
Vapaat asiasanat: epigenetics; hormone; maternal effects; methylation; Parus major; prenatal
Liittyvät organisaatiot
OKM-raportointi: Kyllä
VIRTA-lähetysvuosi: 2023
JUFO-taso: 2
