A1 Journal article (refereed)
Interaction of the C2C12 myotube contractions and glucose availability on transcriptome and extracellular vesicle microRNAs (2024)

Lautaoja-Kivipelto, J. H., Karvinen, S., Korhonen, T.-M., O'Connell, T. M., Tiirola, M., Hulmi, J. J., & Pekkala, S. (2024). Interaction of the C2C12 myotube contractions and glucose availability on transcriptome and extracellular vesicle microRNAs. American Journal of Physiology : Cell Physiology, 326(2), C348-C361. https://doi.org/10.1152/ajpcell.00401.2023

JYU authors or editors

Publication details

All authors or editors: Lautaoja-Kivipelto, Juulia H.; Karvinen, Sira; Korhonen, Tia-Marje; O'Connell, Thomas M; Tiirola, Marja; Hulmi, Juha J.; Pekkala, Satu

Journal or series: American Journal of Physiology : Cell Physiology

ISSN: 0363-6143

eISSN: 1522-1563

Publication year: 2024

Publication date: 04/12/2023

Volume: 326

Issue number: 2

Pages range: C348-C361

Publisher: American Physiological Society

Publication country: United States

Publication language: English

DOI: https://doi.org/10.1152/ajpcell.00401.2023

Publication open access: Not open

Publication channel open access: 

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/92734

Abstract

Exercise-like electrical pulse stimulation (EL-EPS) of myotubes mimics many key physiological changes induced by in vivo exercise. Besides enabling intracellular research, EL-EPS allows to study secreted factors, including muscle-specific microRNAs (myomiRs) carried in extracellular vesicles (EVs). These factors can participate in contraction-induced intercellular crosstalk and may mediate health benefits of exercise. However, the current knowledge of these responses, especially under variable nutritional conditions, is limited. We investigated the effects of EL-EPS on C2C12 myotube transcriptome in high and low glucose conditions by messenger RNA sequencing, while the expression of EV-carried miRNAs was analyzed by small RNA sequencing and RT-qPCR. We show that higher glucose availability augmented contraction-induced transcriptional changes and that the majority of the differentially expressed genes were upregulated. Furthermore, based on the pathway analyses, processes related to contractility and cytokine/inflammatory responses were upregulated. Additionally, we report that EL-EPS increased packing of miR-1-3p into EVs independent of glucose availability. Together our findings suggest that in vitro EL-EPS is a usable tool not only to study contraction-induced intracellular mechanisms, but also extracellular responses. The distinct transcriptional changes observed under variable nutritional conditions emphasize the importance of careful consideration of media composition in future exercise-mimicking studies.

Keywords: muscle cells; extracellular vesicles; microRNA; glucose; cell physiology

Free keywords: electrical pulse stimulation; exercise; exerkine; skeletal muscle

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Ministry reporting: Yes

VIRTA submission year: 2023

Preliminary JUFO rating: 2