A1 Journal article (refereed)
Catalytic Activity of Horseradish Peroxidase Immobilized on Pristine and Two‐Photon Oxidized Graphene (2023)

Schirmer, J., Iatta, E., Emelianov, A. V., Nissinen, M., & Pettersson, M. (2023). Catalytic Activity of Horseradish Peroxidase Immobilized on Pristine and Two‐Photon Oxidized Graphene. Advanced Materials Interfaces, Early View, Article 2300870. https://doi.org/10.1002/admi.202300870

JYU authors or editors

Publication details

All authors or editors: Schirmer, Johanna; Iatta, Ester; Emelianov, Aleksei V.; Nissinen, Maija; Pettersson, Mika

Journal or series: Advanced Materials Interfaces

eISSN: 2196-7350

Publication year: 2023

Publication date: 05/12/2023

Volume: Early View

Article number: 2300870

Publisher: Wiley

Publication country: Germany

Publication language: English

DOI: https://doi.org/10.1002/admi.202300870

Publication open access: Openly available

Publication channel open access: Open Access channel

Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/92521

Abstract

Biosensors based on graphene and bio-graphene interfaces have gained momentum in recent years due to graphene's outstanding electronic and mechanical properties. By introducing the patterning of a single-layer graphene surface by two-photon oxidation (2PO), the surface hydrophobicity/hydrophilicity and doping can be varied at the nanoscale while preserving the carbon network, thus opening possibilities to design new devices. In this study, the effect of 2PO on the catalytic activity of the noncovalently immobilized enzyme horseradish peroxidase (HRP) on single-layer graphene-coated Si/SiO2 chips is presented. To monitor the activity continuously, a simple well-plate setup is introduced. Upon controllable 1–2-layer immobilization, the catalytic activity decreases to a maximum value of 7.5% of the free enzyme. Interestingly, the activity decreases with increasing 2PO area on the samples. Hence, the HRP catalytic activity on the graphene surface is locally controlled. This approach can enable the development of graphene-bio interfaces with locally varying enzyme activity.

Keywords: peroxidases; catalysis; graphene

Contributing organizations

Ministry reporting: Yes

VIRTA submission year: 2023

JUFO rating: 1