A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Catalytic Activity of Horseradish Peroxidase Immobilized on Pristine and Two‐Photon Oxidized Graphene (2023)
Schirmer, J., Iatta, E., Emelianov, A. V., Nissinen, M., & Pettersson, M. (2023). Catalytic Activity of Horseradish Peroxidase Immobilized on Pristine and Two‐Photon Oxidized Graphene. Advanced Materials Interfaces, Early View, Article 2300870. https://doi.org/10.1002/admi.202300870
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Schirmer, Johanna; Iatta, Ester; Emelianov, Aleksei V.; Nissinen, Maija; Pettersson, Mika
Lehti tai sarja: Advanced Materials Interfaces
eISSN: 2196-7350
Julkaisuvuosi: 2023
Ilmestymispäivä: 05.12.2023
Volyymi: Early View
Artikkelinumero: 2300870
Kustantaja: Wiley
Julkaisumaa: Saksa
Julkaisun kieli: englanti
DOI: https://doi.org/10.1002/admi.202300870
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/92521
Tiivistelmä
Biosensors based on graphene and bio-graphene interfaces have gained momentum in recent years due to graphene's outstanding electronic and mechanical properties. By introducing the patterning of a single-layer graphene surface by two-photon oxidation (2PO), the surface hydrophobicity/hydrophilicity and doping can be varied at the nanoscale while preserving the carbon network, thus opening possibilities to design new devices. In this study, the effect of 2PO on the catalytic activity of the noncovalently immobilized enzyme horseradish peroxidase (HRP) on single-layer graphene-coated Si/SiO2 chips is presented. To monitor the activity continuously, a simple well-plate setup is introduced. Upon controllable 1–2-layer immobilization, the catalytic activity decreases to a maximum value of 7.5% of the free enzyme. Interestingly, the activity decreases with increasing 2PO area on the samples. Hence, the HRP catalytic activity on the graphene surface is locally controlled. This approach can enable the development of graphene-bio interfaces with locally varying enzyme activity.
YSO-asiasanat: peroksidaasit; katalyysi; grafeeni
Liittyvät organisaatiot
OKM-raportointi: Kyllä
Raportointivuosi: 2023
Alustava JUFO-taso: 1
