A1 Journal article (refereed)
Antiviral functionalization of cellulose using tannic acid and tannin-rich extracts (2023)


Haapakoski, M., Emelianov, A., Reshamwala, D., Laajala, M., Tienaho, J., Kilpeläinen, P., Liimatainen, J., Jyske, T., Pettersson, M., & Marjomäki, V. (2023). Antiviral functionalization of cellulose using tannic acid and tannin-rich extracts. Frontiers in Microbiology, 14, Article 1287167. https://doi.org/10.3389/fmicb.2023.1287167


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Publication details

All authors or editorsHaapakoski, Marjo; Emelianov, Aleksei; Reshamwala, Dhanik; Laajala, Mira; Tienaho, Jenni; Kilpeläinen, Petri; Liimatainen, Jaana; Jyske, Tuula; Pettersson, Mika; Marjomäki, Varpu

Journal or seriesFrontiers in Microbiology

eISSN1664-302X

Publication year2023

Publication date06/12/2023

Volume14

Article number1287167

PublisherFrontiers Media SA

Publication countrySwitzerland

Publication languageEnglish

DOIhttps://doi.org/10.3389/fmicb.2023.1287167

Publication open accessOpenly available

Publication channel open accessOpen Access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/92511


Abstract

Due to seasonally appearing viruses and several outbreaks and present pandemic, we are surrounded by viruses in our everyday life. In order to reduce viral transmission, functionalized surfaces that inactivate viruses are in large demand. Here the endeavor was to functionalize cellulose-based materials with tannic acid (TA) and tannin-rich extracts by using different binding polymers to prevent viral infectivity of both non-enveloped coxsackievirus B3 (CVB3) and enveloped human coronavirus OC43 (HCoV-OC43). Direct antiviral efficacy of TA and spruce bark extract in solution was measured: EC50 for CVB3 was 0.12 and 8.41 μg/ml and for HCoV-OC43, 78.16 and 95.49 μg/ml, respectively. TA also led to an excellent 5.8- to 7-log reduction of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus infectivity. TA functionalized materials reduced infectivity already after 5-min treatment at room temperature. All the tested methods to bind TA showed efficacy on paperboard with 0.1 to 1% (w/v) TA concentrations against CVB3 whereas material hydrophobicity decreased activities. Specific signatures for TA and HCoV-OC43 were discovered by Raman spectroscopy and showed clear co-localization on the material. qPCR study suggested efficient binding of CVB3 to the TA functionalized cellulose whereas HCoV-OC43 was flushed out from the surfaces more readily. In conclusion, the produced TA-materials showed efficient and broadly acting antiviral efficacy. Additionally, the co-localization of TA and HCoV-OC43 and strong binding of CVB3 to the functionalized cellulose demonstrates an interaction with the surfaces. The produced antiviral surfaces thus show promise for future use to increase biosafety and biosecurity by reducing pathogen persistence.


Keywordscellulosetanninsbarkantimicrobial compoundsenterovirusescoronavirusesRaman spectroscopy

Free keywordsantiviral functionalization; enteroviruses; coronaviruses; tannic acid; cellulose; bark extract; Raman spectroscopy


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Ministry reportingYes

Reporting Year2023

JUFO rating1


Last updated on 2024-15-05 at 13:26