A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Menopausal transition alters female skeletal muscle transcriptome (2024)

Juppi, H.-K., Korhonen, T.-M., Sievänen, T., Kovanen, V., Kujala, U. M., Aukee, P., Cronin, N., Sipilä, S., Karvinen, S., & Laakkonen, E. K. (2024). Menopausal transition alters female skeletal muscle transcriptome. Translational Exercise Biomedicine, 1(1), 43-59. https://doi.org/10.1515/teb-2024-2001

JYU-tekijät tai -toimittajat

Juppi, Hanna-Kaarina
Korhonen, Tia-Marje
Sievänen, Tero
Kovanen, Vuokko
Kujala, Urho
Cronin, Neil
Sipilä, Sarianna
Karvinen, Sira
Laakkonen, Eija

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Juppi, Hanna-Kaarina; Korhonen, Tia-Marje; Sievänen, Tero; Kovanen, Vuokko; Kujala, Urho M.; Aukee, Pauliina; Cronin, Neil; Sipilä, Sarianna; Karvinen, Sira; Laakkonen, Eija K.

Lehti tai sarja: Translational Exercise Biomedicine

eISSN: 2942-6812

Julkaisuvuosi: 2024

Ilmestymispäivä: 13.03.2024

Volyymi: 1

Lehden numero: 1

Artikkelin sivunumerot: 43-59

Kustantaja: Walter de Gruyter GmbH

Julkaisumaa: Saksa

Julkaisun kieli: englanti

DOI: https://doi.org/10.1515/teb-2024-2001

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/94028

Tiivistelmä

Objectives
Although skeletal muscle is a target of hormonal regulation, the muscle transcriptome, including messenger-RNA (mRNA), long non-coding RNAs (lncRNAs), and microRNAs (miRNAs) has not previously been studied across the menopausal transition. Thus, we took a multi-RNA-omics approach to get insight into transcriptome-wide events of menopause.
Methods
We included baseline and follow-up muscle samples from seven early (EarlyMT) and 17 late perimenopausal (LateMT) women transitioning to early postmenopause during the study. Total RNA was sequenced and differential expression (DE) of the transcriptome was investigated. Gene functions were investigated with pathway analyses and protein level expression with Western Blot.
Results
We found 30 DE mRNA genes in EarlyMT and 19 in LateMT participating in pathways controlling cell death, growth, and interactions with the external environment. Lack of protein level changes may indicate a specific role of the regulatory RNAs during menopause. 10 DE lncRNA transcripts but no DE lncRNA genes were identified. No DE miRNAs were found. We identified putative regulatory networks likely to be affected by estradiol availability. Changes in gene expression were correlated with changes in body composition variables, indicating that muscularity and adiposity regulators are affected by menopausal transition. We also found correlations between gene expression and physical activity levels.
Conclusions
The observed DE genes and their regulatory networks offer novel mechanistic insights into factors affecting body composition during and after menopause. Our results imply that physiological deteriorations orchestrated by the muscle transcriptome likely depend on the magnitude of hormonal change and are influenced by physical activity.

YSO-asiasanat: ikääntyminen; naiset; fysiologia; lihakset; hormonaaliset tekijät; estrogeenit; vaihdevuodet; transkriptio (biologia); RNA