A1 Journal article (refereed)
Progression of herpesvirus infection remodels mitochondrial organization and metabolism (2024)
Leclerc, S., Gupta, A., Ruokolainen, V., Chen, J.-H., Kunnas, K., Ekman, A. A., Niskanen, H., Belevich, I., Vihinen, H., Turkki, P., Perez-Berna, A. J., Kapishnikov, S., Mäntylä, E., Harkiolaki, M., Dufour, E., Hytönen, V., Pereiro, E., McEnroe, T., Fahy, K., . . . Vihinen-Ranta, M. (2024). Progression of herpesvirus infection remodels mitochondrial organization and metabolism. PLoS pathogens, 20, Article e1011829. https://doi.org/10.1371/journal.ppat.1011829
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Publication details
All authors or editors: Leclerc, Simon; Gupta, Alka; Ruokolainen, Visa; Chen, Jian-Hua; Kunnas, Kari; Ekman, Axel A.; Niskanen, Henri; Belevich, Ilya; Vihinen, Helena; Turkki, Paula; et al.
Journal or series: PLoS pathogens
ISSN: 1553-7366
eISSN: 1553-7374
Publication year: 2024
Publication date: 15/04/2024
Volume: 20
Article number: e1011829
Publisher: Public Library of Science (PLoS)
Publication country: United States
Publication language: English
DOI: https://doi.org/10.1371/journal.ppat.1011829
Publication open access: Openly available
Publication channel open access: Open Access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/94447
Abstract
Viruses target mitochondria to promote their replication, and infection-induced stress during the progression of infection leads to the regulation of antiviral defenses and mitochondrial metabolism which are opposed by counteracting viral factors. The precise structural and functional changes that underlie how mitochondria react to the infection remain largely unclear. Here we show extensive transcriptional remodeling of protein-encoding host genes involved in the respiratory chain, apoptosis, and structural organization of mitochondria as herpes simplex virus type 1 lytic infection proceeds from early to late stages of infection. High-resolution microscopy and interaction analyses unveiled infection-induced emergence of rough, thin, and elongated mitochondria relocalized to the perinuclear area, a significant increase in the number and clustering of endoplasmic reticulum-mitochondria contact sites, and thickening and shortening of mitochondrial cristae. Finally, metabolic analyses demonstrated that reactivation of ATP production is accompanied by increased mitochondrial Ca2+ content and proton leakage as the infection proceeds. Overall, the significant structural and functional changes in the mitochondria triggered by the viral invasion are tightly connected to the progression of the virus infection.
Keywords: mitochondria; Herpesviridae infections; herpes simplex virus
Free keywords: mitochondria; respiratory infections; apoptosis; herpes simplex virus-1; gene regulation; mitochondrial membrane; viral replication; vero cells
Contributing organizations
Ministry reporting: Yes
Preliminary JUFO rating: 3
