A1 Journal article (refereed)
Triggering Breast Cancer Apoptosis via Cyclin-Dependent Kinase Inhibition and DNA Damage by Novel Pyrimidinone and 1,2,4-Triazolo[4,3-a]pyrimidinone Derivatives (2024)
Abd Al Moaty, M. N., El Kilany, Y., Awad, L. F., Soliman, S. M., Barakat, A., Ibrahim, N. A., Abu-Serie, M. M., Haukka, M., El-Yazbi, A., & Teleb, M. (2024). Triggering Breast Cancer Apoptosis via Cyclin-Dependent Kinase Inhibition and DNA Damage by Novel Pyrimidinone and 1,2,4-Triazolo[4,3-a]pyrimidinone Derivatives. ACS Omega, Early online. https://doi.org/10.1021/acsomega.4c00466
JYU authors or editors
Publication details
All authors or editors: Abd Al Moaty, Mohamed N.; El Kilany, Yeldez; Awad, Laila F.; Soliman, Saied M.; Barakat, Assem; Ibrahim, Nihal A.; Abu-Serie, Marwa M.; Haukka, Matti; El-Yazbi, Amira; Teleb, Mohamed
Journal or series: ACS Omega
ISSN: 2470-1343
eISSN: 2470-1343
Publication year: 2024
Publication date: 06/05/2024
Volume: Early online
Publisher: American Chemical Society (ACS)
Publication country: United States
Publication language: English
DOI: https://doi.org/10.1021/acsomega.4c00466
Publication open access: Openly available
Publication channel open access: Partially open access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/95096
Abstract
Combinations of apoptotic inducers are common clinical practice in breast cancer. However, their efficacy is limited by the heterogeneous pharmacokinetic profiles. An advantageous alternative is merging their molecular entities in hybrid multitargeted scaffolds exhibiting synergistic activities and uniform distribution. Herein, we report apoptotic inducers simultaneously targeting DNA and CDK-2 (cyclin-dependent kinase-2) inspired by studies revealing that CDK-2 inhibition sensitizes breast cancer to DNA-damaging agents. Accordingly, rationally substituted pyrimidines and triazolopyrimidines were synthesized and assayed by MTT against MCF-7, MDA-MB231, and Wi-38 cells compared to doxorubicin. The N-(4-amino-2-((2-hydrazinyl-2-oxoethyl)thio)-6-oxo-1,6-dihydropyrimidin-5-yl)acetamide 5 and its p-nitrophenylhydrazone 8 were the study hits against MCF-7 (IC50 = 0.050 and 0.146 μM) and MDA-MB231 (IC50 = 0.826 and 0.583 μM), induced DNA damage at 10.64 and 30.03 nM, and inhibited CDK-2 (IC50 = 0.172 and 0.189 μM). 5 induced MCF-7 apoptosis by 46.75% and disrupted cell cycle during S phase. Docking and MD simulations postulated their stable key interactions.
Keywords: fluorescence; genetics; mixtures; cancerous diseases
Free keywords: apoptosis; cancer; fluorescence; genetics; mixtures
Contributing organizations
Ministry reporting: Yes
Reporting Year: 2024
Preliminary JUFO rating: 1