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Leveraging the histidine kinase-phosphatase duality to sculpt two-component signaling (2024)

Meier, S. S. M., Multamäki, E., Ranzani, A. T., Takala, H., & Möglich, A. (2024). Leveraging the histidine kinase-phosphatase duality to sculpt two-component signaling. Nature Communications, 15, Article 4876. https://doi.org/10.1038/s41467-024-49251-8

JYU-tekijät tai -toimittajat

Takala, Heikki

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Meier, Stefanie S. M.; Multamäki, Elina; Ranzani, Américo T.; Takala, Heikki; Möglich, Andreas

Lehti tai sarja: Nature Communications

eISSN: 2041-1723

Julkaisuvuosi: 2024

Ilmestymispäivä: 10.06.2024

Volyymi: 15

Artikkelinumero: 4876

Kustantaja: Nature Publishing Group

Julkaisumaa: Britannia

Julkaisun kieli: englanti

DOI: https://doi.org/10.1038/s41467-024-49251-8

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/95905

Tiivistelmä

Bacteria must constantly probe their environment for rapid adaptation, a crucial need most frequently served by two-component systems (TCS). As one component, sensor histidine kinases (SHK) control the phosphorylation of the second component, the response regulator (RR). Downstream responses hinge on RR phosphorylation and can be highly stringent, acute, and sensitive because SHKs commonly exert both kinase and phosphatase activity. With a bacteriophytochrome TCS as a paradigm, we here interrogate how this catalytic duality underlies signal responses. Derivative systems exhibit tenfold higher red-light sensitivity, owing to an altered kinase-phosphatase balance. Modifications of the linker intervening the SHK sensor and catalytic entities likewise tilt this balance and provide TCSs with inverted output that increases under red light. These TCSs expand synthetic biology and showcase how deliberate perturbations of the kinase-phosphatase duality unlock altered signal-response regimes. Arguably, these aspects equally pertain to the engineering and the natural evolution of TCSs.

YSO-asiasanat: bakteerit; soluviestintä; fosforylaatio; fytokromit; reseptorit (biokemia); geeniekspressio; fotobiologia