A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Directional selection, not the direction of selection, affects telomere length and copy number at ribosomal RNA loci (2024)

Sadler, D. E., Watts, P., & Uusi-Heikkilä, S. (2024). Directional selection, not the direction of selection, affects telomere length and copy number at ribosomal RNA loci. Scientific Reports, 14, Article 12162. https://doi.org/10.1038/s41598-024-63030-x

JYU-tekijät tai -toimittajat

Sadler, Daniel
Watts, Phillip
Uusi-Heikkilä, Silva

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Sadler, Daniel E.; Watts, Phillip, C.; Uusi-Heikkilä, Silva

Lehti tai sarja: Scientific Reports

eISSN: 2045-2322

Julkaisuvuosi: 2024

Ilmestymispäivä: 28.05.2024

Volyymi: 14

Artikkelinumero: 12162

Kustantaja: Springer

Julkaisumaa: Britannia

Julkaisun kieli: englanti

DOI: https://doi.org/10.1038/s41598-024-63030-x

Julkaisun avoin saatavuus: Avoimesti saatavilla

Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/95903

Julkaisu on rinnakkaistallennettu: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11130246/

Tiivistelmä

Many fisheries exert directional selection on traits such as body size and growth rate. Whether directional selection impacts regions of the genome associated with traits related to growth is unknown. To address this issue, we characterised copy number variation in three regions of the genome associated with cell division, (1) telomeric DNA, (2) loci transcribed as ribosomal RNA (rDNA), and (3) mitochondrial DNA (mtDNA), in three selection lines of zebrafish reared at three temperatures (22 °C, 28 °C, and 34 °C). Selection lines differed in (1) the direction of selection (two lines experienced directional selection for large or small body size) and (2) whether they experienced any directional selection itself. Lines that had experienced directional selection were smaller, had lower growth rate, shorter telomeres, and lower rDNA copy number than the line that experiencing no directional selection. Neither telomere length nor rDNA copy number were affected by temperature. In contrast, mtDNA content increased at elevated temperature but did not differ among selection lines. Though directional selection impacts rDNA and telomere length, direction of such selection did not matter, whereas mtDNA acts as a stress marker for temperature. Future work should examine the consequences of these genomic changes in natural fish stocks.

YSO-asiasanat: kalakannat; kalatalous; sopeutuminen; fenotyyppi; genotyyppi; muuntelu (biologia); genomiikka; telomeerit; RNA; ribosomit; mitokondrio-DNA