A1 Journal article (refereed)
Glutaraldehyde-enhanced autofluorescence as a general tool for 3D morphological imaging (2024)
Niemeläinen, M., Haapanen-Saaristo, A.-M., Koskinen, L. M., Gullmets, J., Peuhu, E., Meinander, A., Calhim, S., & Paatero, I. (2024). Glutaraldehyde-enhanced autofluorescence as a general tool for 3D morphological imaging. Biology Open, 13(11), Article bio060428. https://doi.org/10.1242/bio.060428
JYU authors or editors
Publication details
All authors or editors: Niemeläinen, Miika; Haapanen-Saaristo, Anna-Mari; Koskinen, Leena M.; Gullmets, Josef; Peuhu, Emilia; Meinander, Annika; Calhim, Sara; Paatero, Ilkka
Journal or series: Biology Open
eISSN: 2046-6390
Publication year: 2024
Publication date: 21/10/2024
Volume: 13
Issue number: 11
Article number: bio060428
Publisher: The Company of Biologists
Publication country: United Kingdom
Publication language: English
DOI: https://doi.org/10.1242/bio.060428
Publication open access: Openly available
Publication channel open access: Open Access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/98491
Abstract
Routine histochemical techniques are capable of producing vast amount of information from diverse samples types, but these techniques are limited in their ability to generate 3D information. Autofluorescence imaging can be used to analyse samples in 3D but it suffers from weak/low signal intensities. Here, we describe a simple chemical treatment with glutaraldehyde to enhance autofluorescence for 3D fluorescence imaging and to generate detailed morphological images on whole-mount samples. This methodology is straightforward and cost-effective to implement, suitable for a wide range of organisms and sample types. Furthermore, it can be readily integrated with standard confocal and fluorescence microscopes for analysis. This approach has the potential to facilitate the analysis of biological 3D structures and research in developmental biology, including studies on model and non-model organisms.
Keywords: Three-dimensional imaging; fluorescence; developmental biology; microscopy
Free keywords: 3D imaging; autofluorescence; developmental biology; microscopy; model organism; non-model organism
Contributing organizations
Ministry reporting: Yes
VIRTA submission year: 2024
Preliminary JUFO rating: 1
