A1 Journal article (refereed)
Fast and cost‐efficient species identification of Atlantic salmon (Salmo salar L.), brown trout (Salmo trutta), and their hybrids using a single SNP marker (2024)
Aykanat, T., Balatsou, A., Kähkönen, K., Syrjänen, J. T., Janhunen, M., Leinonen, T., Prokkola, J. M., Norrgård, J. R., & Piccolo, J. J. (2024). Fast and cost‐efficient species identification of Atlantic salmon (Salmo salar L.), brown trout (Salmo trutta), and their hybrids using a single SNP marker. Journal of Fish Biology, Early online. https://doi.org/10.1111/jfb.16032
JYU authors or editors
Publication details
All authors or editors: Aykanat, Tutku; Balatsou, Athina; Kähkönen, Kirsi; Syrjänen, Jukka T.; Janhunen, Matti; Leinonen, Tuomas; Prokkola, Jenni M.; Norrgård, Johnny R.; Piccolo, John J.
Journal or series: Journal of Fish Biology
ISSN: 0022-1112
eISSN: 1095-8649
Publication year: 2024
Publication date: 17/12/2024
Volume: Early online
Publisher: Wiley
Publication country: United Kingdom
Publication language: English
DOI: https://doi.org/10.1111/jfb.16032
Research data link: https://doi.org/10.6084/m9.figshare.27679287
Publication open access: Openly available
Publication channel open access: Partially open access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/99043
Abstract
A workflow for developing a cost- and time-efficient, single nucleotide polymorphism (SNP)-based assay for species and hybrid identification is described. In a reference set (n = 46), the developed assay identified individuals of two closely related species, the Atlantic salmon (Salmo salar L., n = 23) and brown trout (Salmo trutta, n = 23), with 100% accuracy. Furthermore, species and hybrid identification using field-collected embryos had 98.1% concordance (155/158) to more expensive and time-consuming methods that utilized multiple SNP markers. The method can be integrated into management and conservation plans to quantify species' spawning distribution and hybridization rates.
Keywords: diagnostic methods; determination of species; gene tests; hybridisation; Salmoniformes; nature conservation
Free keywords: conservation genetics; diagnostic locus; hybridization; KASP SNP-assays; species identification
Contributing organizations
Ministry reporting: Yes
Preliminary JUFO rating: 1
