A1 Journal article (refereed)
New formyl indole derivatives based on thiobarbituric acid and their nano-formulations : synthesis, characterization, parasitology and histopathology investigations (2025)


Abdelhalim, W. A., Rabee, A. R., Soliman, S. M., Hagar, M., Moneer, E. A., Bakr, B. A., Barakat, A., Haukka, M., & Rasheed, H. A. (2025). New formyl indole derivatives based on thiobarbituric acid and their nano-formulations : synthesis, characterization, parasitology and histopathology investigations. Scientific Reports, 15, Article 299. https://doi.org/10.1038/s41598-024-81683-6


JYU authors or editors


Publication details

All authors or editorsAbdelhalim, Walaa Ali; Rabee, Ahmed R.; Soliman, Saied M.; Hagar, Mohamed; Moneer, Esraa A.; Bakr, Basant A.; Barakat, Assem; Haukka, Matti; Rasheed, Hanaa A.

Journal or seriesScientific Reports

eISSN2045-2322

Publication year2025

Publication date02/01/2025

Volume15

Article number299

PublisherSpringer Nature

Publication countryUnited Kingdom

Publication languageEnglish

DOIhttps://doi.org/10.1038/s41598-024-81683-6

Publication open accessOpenly available

Publication channel open accessOpen Access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/99323


Abstract

New formyl indole derivatives based on thiobarbituric acid were designed for targeting parasitological applications. The new compounds (5-((1H-indol-3-yl)methylene)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione (3a), and 5-((1-benzyl-1H-indol-3-yl)methylene)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione (3b) were synthesized as thioxodihydropyrimidine derivatives via aldol condensation reaction. The structures of the synthesized compounds were confirmed based on their spectral data via FT-IR, 1H and 13C NMR spectral characterization. In addition, the structure of 3a is confirmed using X-ray crystallography. The synthesized compounds were prepared in nm scale via chitosan as a matrix, and their size was measured via scanning electronic microscope. Interestingly, the newly synthesized nano formulations show higher positive zeta potential (mV) values + 29.6 and + 26.1 for compounds NP-3a, and NP-3b; respectively. These compounds were tested for their parasitological activity. The results revealed that 3b had a great activity against cryptosporidium infection. Moreover, the nano formulation of compound 3b showed a significant reduction percent of oocyst count of cryptosporidium infected mice representing 66%. Furthermore, these compounds were screened by in-vitro hemolytic activity assay (IC50) values (cytotoxicity on RBCs) to assess their cytotoxic potentials and safety profiles.


Keywordsparasitologynanostructureshistologyelectron microscopychemical synthesisinorganic chemistry

Free keywordsthiobarbituric acid; aldol condensation; nano formulation; parasitology; histopathology investigation


Contributing organizations


Ministry reportingYes

VIRTA submission year2025

Preliminary JUFO rating1


Last updated on 2025-25-01 at 20:05