G5 Doctoral dissertation (article)
Towards next-generation peripheral nerve interfaces : tuning graphene field-effect transistor biosensor devices by two-photon oxidation and protein functionalization (2025)
Kohti seuraavan sukupolven ääreishermostollisia rajapintoja : grafeenikanavatransistoribiosensoreiden hienosäätö kaksifotonihapetuksella ja proteiinifunktionalisoinnilla
Lampinen, A. (2025). Towards next-generation peripheral nerve interfaces : tuning graphene field-effect transistor biosensor devices by two-photon oxidation and protein functionalization [Doctoral dissertation]. University of Jyväskylä. JYU Dissertations, 879. https://urn.fi/URN:ISBN:978-952-86-0488-4
JYU authors or editors
Publication details
All authors or editors: Lampinen, Aku
eISBN: 978-952-86-0488-4
Journal or series: JYU Dissertations
eISSN: 2489-9003
Publication year: 2025
Number in series: 879
Number of pages in the book: 1 verkkoaineisto (83 sivua, 106 sivua useina numerointijaksoina, 34 numeroimatonta sivua)
Publisher: University of Jyväskylä
Publication country: Finland
Publication language: English
Persistent website address: https://urn.fi/URN:ISBN:978-952-86-0488-4
Publication open access: Openly available
Publication channel open access: Open Access channel
Abstract
Based on the findings of this work, 2PO was found to be an extremely powerful method of controlling SLG functionalization and modification. 2PO was successfully used to achieve area-selective non-covalent adsorption of different proteins, including those closely related to the function of the nervous system. The adsorption was controlled by varying the oxidation level achieved with 2PO, the pH or protein concentration of the protein incubation solution, and/or the number of incubation cycles performed. Results showed controlled binding ranging from monolayer to multilayer deposition. Additionally, 2PO was used to increase and control the sensitivity of GFETs to pH and [Ca2+]. However, polymer residues from the lithographic fabrication processes were found to affect the GFET response in a similar way as 2PO, but less systematically.
Bare GFETs achieved limits of detection (LoD) for [Ca2+] down to 10 nM and performing non-covalent functionalization of the GFETs with calmodulin (CaM), a protein that selectively binds to Ca2+, the LoD was improved for worse-performing and/or polymer-contaminated devices.
Keywords: graphene; proteins; transmitters (chemical compounds); biosensors; oxidation (active); transistors; doctoral dissertations
Free keywords: field-effect transistor; two-photon oxidation
Contributing organizations
Related projects
- Towards graphene-based neuroprosthetics
- Pettersson, Mika
- Jane and Aatos Erkko Foundation
Ministry reporting: Yes
VIRTA submission year: 2025