A1 Journal article (refereed)
Neural progenitor cell-derived exosomes in ischemia/reperfusion injury in cardiomyoblasts (2025)
Arvola, O., Stigzelius, V., Ampuja, M., & Kivelä, R. (2025). Neural progenitor cell-derived exosomes in ischemia/reperfusion injury in cardiomyoblasts. BMC Neuroscience, 26, Article 11. https://doi.org/10.1186/s12868-025-00931-1
JYU authors or editors
Publication details
All authors or editors: Arvola, Oiva; Stigzelius, Virpi; Ampuja, Minna; Kivelä, Riikka
Journal or series: BMC Neuroscience
eISSN: 1471-2202
Publication year: 2025
Publication date: 05/02/2025
Volume: 26
Article number: 11
Publisher: BioMed Central
Publication country: United Kingdom
Publication language: English
DOI: https://doi.org/10.1186/s12868-025-00931-1
Publication open access: Openly available
Publication channel open access: Open Access channel
Publication is parallel published (JYX): https://jyx.jyu.fi/handle/123456789/100102
Abstract
intervention for acute myocardial infarction. In the adult human brain, vestigial neuronal progenitor stem cells contribute to neuronal repair and recovery following cerebral ischemic injury, an effect modulated by secreted exosomes. Ischemia conditioned neuronal cell derived supernatant and experimental stroke has been shown to be injurious to the heart. However, whether unconditioned neuronal progenitor cell derived-exosomes can instead protect myocardium represents a profound research gap. We investigated the effects of unconditioned neural stem cell derived exosomes as post-injury treatment for cardiomyoblasts from three neuronal culture conditions; adherent cultures, neurosphere cultures and bioreactor cultures. Small extracellular vesicles were enriched with serial ultracentrifugation, validated via nanoparticle tracking analysis, transmission electron microscopy and Western blot analysis prior to utilization as post-injury treatment for H9c2 cardiomyoblasts following oxygen and glucose deprivation. LDH assay was used to assess viability and Seahorse XF high-resolution respirometry analyzer to investigate post-injury cardiomyocyte bioenergetics. We found no evidence that unconditioned neural stem cell derived exosomes are cardiotoxic nor cardioprotective to H9c2 cardiomyoblasts following ischemia-reperfusion injury. Based on our findings, utilizing unconditioned neural stem cell derived exosomes as post-injury treatment for other organs should not have adverse effects to the damaged cardiac cells.
Keywords: stem cells; extracellular vesicles; myocardial infarction; neurons; cardiac muscle cells; biomedicine
Free keywords: neural stem cells; exosome; small EV; stroke; myocardial infarction; IR-injury
Contributing organizations
Ministry reporting: Yes
VIRTA submission year: 2025
Preliminary JUFO rating: 1
