A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Genomic evolution of bacterial populations under co-selection by antibiotics and phage (2017)
Cairns, J., Frickel, J., Jalasvuori, M., Hiltunen, T., & Becks, L. (2017). Genomic evolution of bacterial populations under co-selection by antibiotics and phage. Molecular Ecology, 26(7), 1848-1859. https://doi.org/10.1111/mec.13950
JYU-tekijät tai -toimittajat
Julkaisun tiedot
Julkaisun kaikki tekijät tai toimittajat: Cairns, Johannes; Frickel, Jens; Jalasvuori, Matti; Hiltunen, Teppo; Becks, Lutz
Lehti tai sarja: Molecular Ecology
ISSN: 0962-1083
eISSN: 1365-294X
Julkaisuvuosi: 2017
Volyymi: 26
Lehden numero: 7
Artikkelin sivunumerot: 1848-1859
Kustantaja: Wiley-Blackwell Publishing Ltd.
Julkaisumaa: Britannia
Julkaisun kieli: englanti
DOI: https://doi.org/10.1111/mec.13950
Linkki tutkimusaineistoon: http://dx.doi.org/10.5061/dryad.62s22
Julkaisun avoin saatavuus: Ei avoin
Julkaisukanavan avoin saatavuus:
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/53814
Lisätietoja: Special Issue: Microbial Local Adaptation
Tiivistelmä
Bacteria live in dynamic systems where selection pressures can alter rapidly, forcing adaptation to the prevailing conditions. In particular, bacteriophages and antibiotics of anthropogenic origin are major bacterial stressors in many environments. We previously observed that populations of the bacterium Pseudomonas fluorescens SBW25 exposed to the lytic bacteriophage SBW25Φ2 and a noninhibitive concentration of the antibiotic streptomycin (coselection) achieved higher levels of phage resistance compared to populations exposed to the phage alone. In addition, the phage became extinct under coselection while remaining present in the phage alone environment. Further, phenotypic tests indicated that these observations might be associated with increased mutation rate under coselection. In this study, we examined the genetic causes behind these phenotypes by whole‐genome sequencing clones isolated from the end of the experiments. We were able to identify genetic factors likely responsible for streptomycin resistance, phage resistance and hypermutable (mutator) phenotypes. This constitutes genomic evidence in support of the observation that while the presence of phage did not affect antibiotic resistance, the presence of antibiotic affected phage resistance. We had previously hypothesized an association between mutators and elevated levels of phage resistance under coselection. However, our evidence regarding the mechanism was inconclusive, as although with phage mutators were only found under coselection, additional genomic evidence was lacking and phage resistance was also observed in nonmutators under coselection. More generally, our study provides novel insights into evolution between univariate and multivariate selection (here two stressors), as well as the potential role of hypermutability in natural communities.
YSO-asiasanat: antibioottiresistenssi
Vapaat asiasanat: experimental evolution; sublethal antibiotic concentrations; phage resistance; Pseudomonas fluorescens; phage phi-2
Liittyvät organisaatiot
OKM-raportointi: Kyllä
Raportointivuosi: 2017
JUFO-taso: 2
