A1 Journal article (refereed)
Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium (2017)

Ashrafi, R., Bruneaux, M., Sundberg, L.-R., Pulkkinen, K., & Ketola, T. (2017). Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium. Scientific Reports, 7, Article 980. https://doi.org/10.1038/s41598-017-01074-y

JYU authors or editors

Publication details

All authors or editorsAshrafi, Roghaieh; Bruneaux, Matthieu; Sundberg, Lotta-Riina; Pulkkinen, Katja; Ketola, Tarmo

Journal or seriesScientific Reports



Publication year2017


Issue number0

Article number980

PublisherNature Publishing Group

Publication countryUnited Kingdom

Publication languageEnglish


Research data linkhttps://jyx.jyu.fi/dspace/handle/123456789/53541

Publication open accessOpenly available

Publication channel open accessOpen Access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/53693


Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate change projections. Many microbes rely on strong interference competition; hence the fitness of many pathogenic bacteria could be a function of both their growth properties and intraspecific competition. However, due to technical challenges in distinguishing and tracking individual strains, experimental evidence on intraspecific competition has been limited so far. Here, we developed a robust application of the high-resolution melting (HRM) assay to study head-to-head competition between mixed genotype co-cultures of a waterborne bacterial pathogen of fish, Flavobacterium columnare, at two different temperatures. We found that competition outcome in liquid cultures seemed to be well predicted by growth yield of isolated strains, but was mostly inconsistent with interference competition results measured in inhibition tests on solid agar, especially as no growth inhibition between strain pairs was detected at the higher temperature. These results suggest that, for a given temperature, the factors driving competition outcome differ between liquid and solid environments.


Free keywordspathogenic bacteria; intraspecific competition; high-resolution melting (HRM) assay

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Ministry reportingYes

Reporting Year2017

JUFO rating2

Last updated on 2024-08-01 at 16:07