A1 Journal article (refereed)
Skeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing (2017)


Seppälä, J., Bernardi, R. C., Haataja, T., Hellman, M., Pentikäinen, O., Schulten, K., Permi, P., Ylänne, J., & Pentikäinen, U. (2017). Skeletal Dysplasia Mutations Effect on Human Filamins’ Structure and Mechanosensing. Scientific Reports, 7, Article 4218. https://doi.org/10.1038/s41598-017-04441-x


JYU authors or editors


Publication details

All authors or editorsSeppälä, Jonne; Bernardi, Rafael C.; Haataja, Tatu; Hellman, Maarit; Pentikäinen, Olli; Schulten, Klaus; Permi, Perttu; Ylänne, Jari; Pentikäinen, Ulla

Journal or seriesScientific Reports

ISSN2045-2322

eISSN2045-2322

Publication year2017

Volume7

Issue number0

Article number4218

PublisherNature Publishing Group

Publication countryUnited Kingdom

Publication languageEnglish

DOIhttps://doi.org/10.1038/s41598-017-04441-x

Persistent website addresshttps://www.nature.com/articles/s41598-017-04441-x.pdf

Publication open accessOpenly available

Publication channel open accessOpen Access channel

Publication is parallel published (JYX)https://jyx.jyu.fi/handle/123456789/54830


Abstract

Cells’ ability to sense mechanical cues in their environment is crucial for fundamental cellular processes, leading defects in mechanosensing to be linked to many diseases. The actin cross-linking protein Filamin has an important role in the conversion of mechanical forces into biochemical signals. Here, we reveal how mutations in Filamin genes known to cause Larsen syndrome and Frontometaphyseal dysplasia can affect the structure and therefore function of Filamin domains 16 and 17. Employing X-ray crystallography, the structure of these domains was first solved for the human Filamin B. The interaction seen between domains 16 and 17 is broken by shear force as revealed by steered molecular dynamics simulations. The effects of skeletal dysplasia associated mutations of the structure and mechanosensing properties of Filamin were studied by combining various experimental and theoretical techniques. The results showed that Larsen syndrome associated mutations destabilize or even unfold domain 17. Interestingly, those Filamin functions that are mediated via domain 17 interactions with other proteins are not necessarily affected as strongly interacting peptide binding to mutated domain 17 induces at least partial domain folding. Mutation associated to Frontometaphyseal dysplasia, in turn, transforms 16–17 fragment from compact to an elongated form destroying the force-regulated domain pair.


KeywordsNMR spectroscopyx-ray crystallography

Free keywordscomputational biophysics; cytoskeletal proteins; SAXS


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Ministry reportingYes

Reporting Year2017

JUFO rating2


Last updated on 2024-08-01 at 19:54