A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Exploring fast proton transfer events associated with lateral proton diffusion on the surface of membranes (2019)

Amdursky, N., Lin, Y., Aho, N., & Groenhof, G. (2019). Exploring fast proton transfer events associated with lateral proton diffusion on the surface of membranes. Proceedings of the National Academy of Sciences, 116(7), 2443-2451. https://doi.org/10.1073/pnas.1812351116

JYU-tekijät tai -toimittajat

Aho, Noora
Groenhof, Gerrit

Julkaisun tiedot

Julkaisun kaikki tekijät tai toimittajat: Amdursky, Nadav; Lin, Yiyang; Aho, Noora; Groenhof, Gerrit

Lehti tai sarja: Proceedings of the National Academy of Sciences

ISSN: 0027-8424

eISSN: 1091-6490

Julkaisuvuosi: 2019

Volyymi: 116

Lehden numero: 7

Artikkelin sivunumerot: 2443-2451

Kustantaja: National Academy of Sciences

Julkaisumaa: Yhdysvallat (USA)

Julkaisun kieli: englanti

DOI: https://doi.org/10.1073/pnas.1812351116

Julkaisun avoin saatavuus: Ei avoin

Julkaisukanavan avoin saatavuus:

Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/64179

Tiivistelmä

Proton diffusion (PD) across biological membranes is a fundamental process in many biological systems, and much experimental and theoretical effort has been employed for deciphering it. Here, we report on a spectroscopic probe, which can be tightly tethered to the membrane, for following fast (nanosecond) proton transfer events on the surface of membranes. Our probe is composed of a photoacid that serves as our light-induced proton source for the initiation of the PD process. We use our probe to follow PD, and its pH dependence, on the surface of lipid vesicles composed of a zwitterionic headgroup, a negative headgroup, a headgroup that is composed only from the negative phosphate group, or a positive headgroup without the phosphate group. We reveal that the PD kinetic parameters are highly sensitive to the nature of the lipid headgroup, ranging from a fast lateral diffusion at some membranes to the escape of protons from surface to bulk (and vice versa) at others. By referring to existing theoretical models for membrane PD, we found that while some of our results confirm the quasi-equilibrium model, other results are in line with the nonequilibrium model.

YSO-asiasanat: molekyylidynamiikka; lipidit; diffuusio (fysikaaliset ilmiöt); protonit

Vapaat asiasanat: kalvot (orgaaniset objektit)

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